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Article Abstract
The molecular mechanisms underlying differences in somatic cell cloning efficiency among buffalo fetal fibroblasts (BFFs) from different individuals remain poorly understood. Our study conducted a comparative analysis of mitochondrial function and chromatin remodeling capacity of BFFs from different individuals, and tracked the mitochondria in the embryos derived from SCNT and IVF. These findings revealed that BFFs with high cloning efficiency displayed well-preserved mitochondrial morphology and ultrastructure, increased mitochondrial DNA copy number and ATP levels, elevated antioxidant capacity, enhanced mitochondrial membrane potential, and significantly upregulated expression levels of mitochondria-related genes. Meanwhile, BFFs with high cloning efficiency demonstrated significantly higher DNA enrichment, lower heterochromatin levels, and differential expression levels of chromatin remodeling-related genes. Furthermore, the persistence of donor cell mitochondria during cloned embryo development was observed, whereas sperm-derived mitochondria during IVF embryo development were rarely detectable. Collectively, our results suggest a tight connection between the mitochondria and chromatin remodeling of donor cells, and demonstrating their synergistic impact on cloning efficiency, providing the crucial experimental evidence for nucleo-mitochondrial interactions.
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| http://dx.doi.org/10.1016/j.theriogenology.2025.117602 | DOI Listing |
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