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http://dx.doi.org/10.1093/gbe/evaf145 | DOI Listing |
J Agric Food Chem
September 2025
School of Chemical Engineering and Technology, Zhengzhou University, Zhengzhou 450001, China.
d-Amino acid oxidase from (DAAO) is valuable for pharmaceutical and chemical synthesis due to its high enantioselectivity, but its poor thermostability limits extensive application. This study proposed a synergistic strategy of "sequence consensus design coupled with structure modification" to enhance DAAO thermostability. Through homologous sequence analysis and greedy algorithm-based optimization, a triple mutant M3 (S18T/V7I/Y132F) was obtained, showing a 3.
View Article and Find Full Text PDFMedicine (Baltimore)
September 2025
Division of General Internal Medicine, Department of Medicine, University Hospitals, Genève, Switzerland.
Rationale: This case highlights the importance of considering a wide range of possible diagnoses when faced with unexplained hemorrhagic symptoms. When standard investigations fail to identify a clear cause, it is essential to conduct a detailed dietary history. This can lead to the diagnosis of scurvy, a reversible vitamin C deficiency that is often overlooked in populations at risk.
View Article and Find Full Text PDFMol Ther Methods Clin Dev
September 2025
Molecular Biology Interdepartmental Program, University of California, Los Angeles, Los Angeles, CA 90095, USA.
X-linked agammaglobulinemia (XLA) is a rare inborn error of immunity caused by loss-of-function mutations in the gene encoding Bruton's tyrosine kinase (BTK). XLA patients lack mature B cells and have negligible antibody levels, leaving them susceptible to recurrent bacterial and chronic viral infections. Autologous hematopoietic stem cell gene therapy with gene-corrected HSC may serve as a promising treatment of XLA; this therapy would provide a one-time cure and would replace lifelong immunoglobulin replacement therapy.
View Article and Find Full Text PDFBiochem Pharmacol
September 2025
School of Basic Medicine, Medical College of Qingdao University, Qingdao 266071, China. Electronic address:
Parkinson's Disease (PD), the second most common neurodegenerative disease after Alzheimer's disease, is clinically characterized by resting tremor, rigidity and postural balance disorder. Its pathological essence is the progressive degenerative death of dopaminergic neurons in the substantia nigra pars compacta (SNpc), leading to a significant decrease in striatal dopamine (DA) levels. This results in the dysfunction of basal ganglia-thalamus-cortex (BGTC) circuit.
View Article and Find Full Text PDFComput Med Imaging Graph
August 2025
Institute of Advanced Technology, Zhejiang University of Technology, Hangzhou, China. Electronic address:
The segmentation of cranial nerves (CNs) tract provides a valuable quantitative tool for the analysis of the morphology and trajectory of individual CNs. Multimodal CN segmentation networks, e.g.
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