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Article Abstract
Aflatoxin B (AFB) is a serious mycotoxin that contaminates food and poses a major threat to global health. In this study, a novel ECL immunosensor platform was developed utilizing HTCPE-TiO as a luminescent probe and PdCu nanoflowers (PdCu NFs) as an electrode substrate. The aggregation-induced enhanced electrochemiluminescence effect (AIECL) and the amplification effect of TiO as a co-reaction promoter were combined in the HTCPE-TiO nanopolymer resulting in a strong ECL response. Coral-like PdCu NFs were used as substrate materials, providing a large surface area that facilitated efficient AFB antigen immobilization and enhanced the sensitivity of the immunosensor. The constructed ECL immunosensor platform demonstrated high sensitivity and selectivity for the quantitative detection of AFB in food samples. The detection range was 0.003-1000 ng mL with a limit of detection of 0.98 pg mL. Moreover, it showed great potential for application in the detection of real samples.
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| http://dx.doi.org/10.1016/j.talanta.2025.128602 | DOI Listing |
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