E3RC: A step-by-step computational protocol for exploring enhancer RNA expression and regulation using conventional RNA-seq data.

STAR Protoc

Westlake Genomics and Bioinformatics Lab, Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou 310024, China; School of Life Sciences, Westlake University, Hangzhou 310024, China; Westlake Institute for Advanced Study, Hangzhou 310024, China. Electronic address:

Published: July 2025


Category Ranking

98%

Total Visits

921

Avg Visit Duration

2 minutes

Citations

20

Article Abstract

Here, we present a computational framework, E3RC, for exploring enhancer RNA (eRNA) expression and regulation using conventional RNA sequencing (RNA-seq) data. Using a public dataset as an example, we describe step-by-step guidelines for identifying eRNAs and characterizing their expressional changes and transcriptional regulation across multiple developmental stages of mouse preimplantation embryos. For complete details on the use and execution of this protocol, please refer to Yu et al..

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12318280PMC
http://dx.doi.org/10.1016/j.xpro.2025.103989DOI Listing

Publication Analysis

Top Keywords

exploring enhancer
8
enhancer rna
8
expression regulation
8
regulation conventional
8
rna-seq data
8
e3rc step-by-step
4
step-by-step computational
4
computational protocol
4
protocol exploring
4
rna expression
4

Similar Publications

Ultrasonographic Analysis of Site-Specific Plantar Skin Thickness for Melanoma Staging and Excision.

Clin Anat

September 2025

Division in Anatomy and Developmental Biology, Department of Oral Biology, Human Identification Research Institute, BK21 FOUR Project, Yonsei University College of Dentistry, Seoul, South Korea.

Plantar melanomas present unique diagnostic and surgical challenges owing to substantial regional variations in skin thickness. Although the Breslow thickness remains the primary criterion for staging and surgical excision, its application on plantar melanoma is complicated by the inherent thickness of the glabrous plantar epidermis, which may lead to tumor depth overestimation. Accurate assessment of plantar skin thickness is essential for optimizing staging accuracy and refining surgical margins.

View Article and Find Full Text PDF

Neuroimaging Data Informed Mood and Psychosis Diagnosis Using an Ensemble Deep Multimodal Framework.

Hum Brain Mapp

September 2025

Tri-Institutional Center for Translational Research in Neuroimaging and Data Science (TReNDS), Georgia State University, Georgia Institute of Technology, and Emory University, Atlanta, Georgia, USA.

Investigating neuroimaging data to identify brain-based markers of mental illnesses has gained significant attention. Nevertheless, these endeavors encounter challenges arising from a reliance on symptoms and self-report assessments in making an initial diagnosis. The absence of biological data to delineate nosological categories hinders the provision of additional neurobiological insights into these disorders.

View Article and Find Full Text PDF

Unlabelled: This report provides a detailed analysis of a singular case involving cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) in a male patient who suffered a stroke. Our investigation delves into the clinical manifestations, genetic foundations, diagnostic complexities, and prognosis associated with CADASIL. As a notable contributor to stroke occurrence in young patients, CADASIL's impact on morbidity and mortality is influenced by stroke-related complications and cognitive decline.

View Article and Find Full Text PDF

Plants, Pills, and the Brain: Exploring Phytochemicals and Neurological Medicines.

Int J Plant Anim Environ Sci

August 2025

Department of Translational Research, College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, Pomona, CA 91766, USA.

Neurological disorders, such as Alzheimer's disease, Parkinson's disease, epilepsy, spinal cord injuries, and traumatic brain injuries, represent substantial global health challenges due to their chronic and often progressive nature. While allopathic medicine offers a range of pharmacological interventions aimed at managing symptoms and mitigating disease progression, it is accompanied by limitations, including adverse side effects, the development of drug resistance, and incomplete efficacy. In parallel, phytochemicals-bioactive compounds derived from plants-are receiving increased attention for their potential neuroprotective, antioxidant, and anti-inflammatory properties.

View Article and Find Full Text PDF

PRMT1-Mediated PARP1 Methylation Drives Lung Metastasis and Chemoresistance via P65 Activation in Triple-Negative Breast Cancer.

Research (Wash D C)

September 2025

State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University Cancer Center, Guangzhou 510060, China.

Triple-negative breast cancer (TNBC) is the most aggressive breast cancer subtype, characterized by a high propensity for metastasis, poor prognosis, and limited treatment options. Research has demonstrated a substantial correlation between the expression of protein arginine N-methyltransferase 1 (PRMT1) and enhanced proliferation, metastasis, and poor outcomes in TNBC. However, the specific role of PRMT1 in lung metastasis and chemoresistance remains unclear.

View Article and Find Full Text PDF