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Article Abstract
is a pathogenic bacterial agent that causes meningococcal meningitis in humans. Developing a rapid and low-cost detection method is crucial, especially for developing countries. This study focuses on the development of an efficient loop-mediated isothermal amplification (LAMP) method for accurate identification. A new LAMP primer set was designed, and a LAMP reaction was optimized. The colorimetric detection method was also applied, and the assay characteristics were evaluated using clinical samples. The results demonstrated a specific LAMP reaction for detection of genotypes A, B, and C, with a limit of detection of 10 cfu/mL, 100% specificity and sensitivity, and a rapid detection time of only 40 min by colorimetric visual inspection. No cross-reactivity with reference strains of , , , , and type b was observed in the LAMP reaction with the new primer set. This result suggests that the LAMP reaction could be a promising tool for developing a rapid detection method suitable for use in Vietnam and other developing countries.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12191605 | PMC |
| http://dx.doi.org/10.3390/cimb47060467 | DOI Listing |
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