Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Microglia are crucial for brain immunity, and their phenotypic changes have been implicated in neuroinflammation and the development of depression and anxiety symptoms. The accurate characterization of these changes is challenging due to the interaction of microglia with the brain microenvironment and the presence of central nervous system (CNS)-resident macrophages that share common markers with microglia. Using the microglia-specific marker TMEM119 and bone marrow chimeric mice, we investigated the changes in microglial activity in mice exposed to chronic corticosterone, a widely used rodent model of stress-related behaviors. Flow cytometry analysis was used to identify TMEM119, CD45 microglia and TMEM119, CD45 microglia. Notably, corticosterone exposure led to a significant increase in the number of TMEM119, CD45 microglia, whereas the proportions of TMEM119, CD45 microglia remained unchanged. The increased forward and side scatter properties of TMEM119, CD45 microglia suggest increased intracellular granularity, which may reflect distinct phenotypic features of this previously overlooked cell population. Our finding of an increased population of TMEM119, CD45 microglia may reflect a stress-adapted phenotype similar to that of clinical depression.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.brainres.2025.149787 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Microglia exhibit a dynamic response, modulating inducible nitric oxide synthase expression and the production of pro-inflammatory cytokines during experimental cerebral malaria.
Front Immunol
August 2025
Laboratório de Pesquisa em Malária, Instituto Oswaldo Cruz and Centro de Pesquisa, Diagnóstico e Treinamento em Malária (CPD-Mal) of Fundação Oswaldo Cruz (Fiocruz) and of Secretaria de Vigilância em Saúde (SVS), Ministério da Saúde, Rio de Janeiro, Brazil.
Microglia play a fundamental role in maintaining central nervous system homeostasis by monitoring brain tissue for physical, structural, and biochemical alterations. Its involvement in the pathogenesis of various neurological disorders is well documented. However, the role of microglia in cerebral malaria, a disease associated with high mortality and long-term neurological sequelae, remains poorly understood.
View Article and Find Full Text PDFTMEM119-defined brain macrophage phenotypes in a chronic stress model induced by corticosterone in male mice.
Brain Res
September 2025
Department of Psychiatry, The National Defense Medical College, Saitama, Japan. Electronic address:
Microglia are crucial for brain immunity, and their phenotypic changes have been implicated in neuroinflammation and the development of depression and anxiety symptoms. The accurate characterization of these changes is challenging due to the interaction of microglia with the brain microenvironment and the presence of central nervous system (CNS)-resident macrophages that share common markers with microglia. Using the microglia-specific marker TMEM119 and bone marrow chimeric mice, we investigated the changes in microglial activity in mice exposed to chronic corticosterone, a widely used rodent model of stress-related behaviors.
View Article and Find Full Text PDFMethamphetamine Induces Systemic Inflammation and Anxiety: The Role of the Gut-Immune-Brain Axis.
Int J Mol Sci
September 2022
Institute for Health and Sport, Victoria University, Melbourne, VIC 3021, Australia.
Methamphetamine (METH) is a highly addictive drug abused by millions of users worldwide, thus becoming a global health concern with limited management options. The inefficiency of existing treatment methods has driven research into understanding the mechanisms underlying METH-induced disorders and finding effective treatments. This study aims to understand the complex interactions of the gastrointestinal-immune-nervous systems following an acute METH dose administration as one of the potential underlying molecular mechanisms concentrating on the impact of METH abuse on gut permeability.
View Article and Find Full Text PDFFlow cytometry analysis of immune and glial cells in a trigeminal neuralgia rat model.
Sci Rep
December 2021
Public Technology Service Center of Fujian Medical University; Laboratory of Clinical Applied Anatomy, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, 350122, P.R. China.
Microvascular compression of the trigeminal root entry zone (TREZ) is the main cause of most primary trigeminal neuralgia (TN), change of glial plasticity was previously studied in the TREZ of TN rat model induced by chronic compression. To better understand the role of astrocytes and immune cells in the TREZ, different cell markers including glial fibrillary acidic protein (GFAP), complement C3, S100A10, CD45, CD11b, glutamate-aspartate transporter (GLAST), Iba-1 and TMEM119 were used in the TN rat model by immunohistochemistry and flow cytometry. On the post operation day 28, GFAP/C3-positive A1 astrocytes and GFAP/S100A10-positive A2 astrocytes were activated in the TREZ after compression injury, there were no statistical differences in the ratios of A1/A2 astrocytes between the sham and TN groups.
View Article and Find Full Text PDFA modified protocol for isolation of retinal microglia from the pig.
Exp Eye Res
June 2021
University of Münster Medical School, Department of Ophthalmology, Münster, Germany. Electronic address:
Microglia are the resident immune cells in the retina. To investigate their properties and behaviour, a reliable and yielding procedure to culture them is necessary. We here describe a way of isolation of microglial cells from the porcine retina, as pig eyes are similar to human eyes in size, structure and vasculature, including similarities in proteins and pathways.
View Article and Find Full Text PDF