Role of Rs228648 and Rs2890565 single nucleotide polymorphisms in Urotensin-2 gene in prostate cancer.

Background: Urotensin-II (U-II), a somatostatin-like cyclic undecapeptide has been the center of many studies due to its effect both on physiological and pathophysiological regulation of many diseases. Recently, U-II has been implicated in the development of breast cancer through its mitogenic and angiogenic actions. Over the years, single nucleotide polymorphisms (SNPs) in the Urotensin II (UTS2) gene, notably rs2890565 (S89N, 3836 C > G/T) and rs228648 (T21M, 143G > A), have been identified. While the correlation of these polymorphisms with breast cancer has been established, their link to prostate cancer (PCa) remains unclear. This study explores the association of UTS2 gene rs2890565 and rs228648 polymorphisms with PCa susceptibility and metastasis.

Methods And Results: A total of 141 healthy volunteers and 158 PCa patients were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), confirmed by Sanger sequencing. No statistically significant association (p > 0.05) was found between either UTS2 gene rs2890565 or rs228648 polymorphisms and the risk of PCa development, Gleason score and tumor and metastatic stage under dominant and recessive genetic models in the Turkish population.

Conclusion: The uniform distribution of SNP genotypes between the patient and healthy volunteers suggests that regardless of our study population size, neither rs2890565 nor rs228648 UTS2 gene polymorphisms are associated with PCa susceptibility and progression in the Turkish population.