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On the 50th anniversary of the discovery of surface-enhanced Raman spectroscopy (SERS), numerous reviews highlighted SERS advancements from different aspects, such as the historical evolution, enhancement mechanisms, quantitative analysis, and medical applications. However, how to develop the rapid SERS analysis for real samples has rarely been summarized yet. This review highlights the following three pivotal steps in this direction: (1) establishing reliable and highly selectively sensitive SERS analysis in the laboratory; (2) developing rapid sample pretreatment; and (3) AI-enhanced qualitative and quantitative SERS analysis.
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http://dx.doi.org/10.1021/acsami.5c05699 | DOI Listing |
Anal Sci
September 2025
Department of Chemistry, Graduate School of Advanced Science and Engineering, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, 739-8526, Japan.
Surface-enhanced Raman scattering (SERS) is a powerful analytical technique; however, its quantitative application has been limited by the instability of substrates and significant signal fluctuations. In this study, we demonstrated that 4-aminobenzenethiol (4-ATP) can be quantitatively detected through statistical analysis of SERS signal intensity distributions obtained using citrate-stabilized AuNPs, biotin-functionalized AuNPs, and gold nanoparticle (AuNP)-bound polystyrene (PS) microparticles. Raman spectra obtained in bulk aqueous solution under static conditions showed that the detection sensitivity of 4-ATP using AuNP-bound PS microparticles was approximately twice that achieved with citrate-stabilized AuNPs or biotin-modified AuNPs.
View Article and Find Full Text PDFMikrochim Acta
September 2025
Teaching & Research Department of Common Course, Shenyang Sport University, Shenyang, 110115, China.
A surface enhanced Raman scattering (SERS)-based sensing platform is devised integrating a TMB redox system for rapid dopamine detection. Gold nanobipyramids (Au NBPs), synthesized via the heat-mediated seed-mediated growth method, possess dual functionality of peroxidase-like activity and SERS activity. This enables them to catalyze the oxidation of TMB and simultaneously amplify the Raman signal of the oxidized TMB product (oxTMB).
View Article and Find Full Text PDFTalanta
September 2025
Faculty of Applied Sciences, Ton Duc Thang University, Ho Chi Minh City, Viet Nam. Electronic address:
Food spoilage poses a global challenge with far-reaching consequences for public health, economic stability, and environmental sustainability. Conventional analytical methods for spoilage detection though accurate are often cost-prohibitive, labor-intensive, and unsuitable for real-time or field-based monitoring. Microfluidic paper-based analytical devices (μPADs) have emerged as a transformative technology offering rapid, portable, and cost-effective solutions for food quality assessment.
View Article and Find Full Text PDFACS Appl Mater Interfaces
September 2025
College of Engineering and Applied Sciences, Nanjing University, Nanjing 210093, P. R. China.
Exhaled breath analysis offers noninvasive, early lung cancer detection via volatile organic compound (VOC) biomarkers, surpassing blood-based methods. Surface-enhanced Raman spectroscopy (SERS) is ideal for this purpose, combining molecular fingerprint specificity with single-molecule sensitivity. However, conventional SERS substrates face a fundamental limitation: while porous materials such as metal-organic frameworks effectively adsorb VOCs through their subnanometer pores (0.
View Article and Find Full Text PDFAnal Chim Acta
November 2025
Measurement Technology & Instrumentation Key Laboratory of Hebei Province, Institute of Electrical Engineering, Yanshan University, Qinhuangdao, 066004, China.
Background: With the development of modern agriculture, neonicotinoid pesticides have been widely used due to their high efficiency and strong systemic properties. However, excessive use leads to the accumulation of residues in the food chain, threatening the ecosystem and human health. Pesticide residues are easily accumulated in oilseed crops and become concentrated during the edible oil refining process.
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