Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Objective: Blackleg, also known as black quarter (BQ), is an acute and lethal infection in bovines and caused by the anaerobic bacterium- Clostridium chauvoei. A killed vaccine is available for disease control, and evaluating the immune response to the vaccine or natural infection in susceptible animals is crucial for implementing effective vaccination strategies in endemic regions. This study focused on the development and comparative evaluation of indirect ELISAs based on native antigens (whole cell and flagellar) from C. chauvoei to detect blackleg-specific antibodies in serum samples collected from cattle.
Methods: Whole cell and flagellar antigens were extracted conventionally from C. chauvoei strain NIVEDIBQ1, and optimized indirect ELISAs were compared with the indirect hemagglutination assay (IHA). These assays were used to screen 810 serum samples collected from Andhra Pradesh, India, where blackleg is endemic.
Results: Optimum antigen concentrations (250 ng of whole cell antigen and 300 ng of flagella), serum dilution (1:100), and conjugate dilution (1: 10,000) were determined using the checkerboard titration method. The optimized assays reliably detected C. chauvoei-specific antibodies at serum dilutions as low as 1:1600 for whole cell antigens, and 1: 800 for flagellar antigens without any cross-reactions. A comparison between IHA and indirect ELISAs using Cohen's Kappa analysis indicated that the two assays were equally effective. A diagnostic sensitivity (DSn) of 96.0 % and 95.0 %, and diagnostic specificity (DSp) of 95.0 % and 97.0 %, with cut-off criteria of >20.7632 and 18.579, for whole cell and flagellar antigens, respectively, was observed after ROC analysis. The observed seropositivity rates in Andhra Pradesh were 57 % (462) and 40.9 % (332) for whole cell and flagellar antigen based indirect ELISAs, respectively.
Conclusion: This study demonstrated the potential application of native antigen-based indirect ELISAs for monitoring blackleg-specific antibodies in cattle from endemic regions.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.anaerobe.2025.102975 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Reducing allergenicity of Trachinotus ovatus parvalbumin: Insights into digestibility and IgE-binding ability after dense phase CO₂ treatment.
Int J Biol Macromol
September 2025
College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Key Labo
Parvalbumin (PV), a thermostable and digestion-resistant fish allergen, has been shown to retain its allergenic potential following traditional treatments, thus posing a persistent allergic risk. The study investigated the digestive kinetics and IgE immunoreactivity of Trachinotus ovatus PV, a major fish allergen, under different treatments (untreated; DPCD treatment-15 MPa, 30 min, 50 °C; heat treatment), to evaluate its allergenic potential alterations. The analysis was conducted using a combination of techniques to assess the proteolytic stability and IgE-binding capacity of PV, including Tris-Tricine-SDS-PAGE, Western blot (WB), indirect enzyme-linked immunosorbent assay (ELISA), and free amino group quantification.
View Article and Find Full Text PDFFirst Report of in Bats and Ticks Collected from Bat Habitats.
Vector Borne Zoonotic Dis
September 2025
Laboratory of Veterinary Internal Medicine, BK21 FOUR Future Veterinary Medicine Leading Education and Research Center, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
The zoonotic infectious disease, severe fever with thrombocytopenia syndrome (SFTS), caused by the SFTS virus (SFTSV), , was first identified in China in 2009 and reported in the Republic of Korea in 2013. The primary vector is the tick (.) , which is endemic to the Asia-Pacific region and has a wide range of hosts.
View Article and Find Full Text PDFRecent advances in techniques used in the diagnosis of lumpy skin disease: a review.
J Vet Diagn Invest
September 2025
Biology Department; Faculty of Sciences, Umm Al-Qura University, Makkah, Saudi Arabia.
Lumpy skin disease (LSD) is a viral disease that affects livestock and is caused by the lumpy skin disease virus (LSDV). An outbreak of LSD in any country can lead to acute economic damage for livestock owners. The significance of prompt and accurate diagnosis in managing this viral disease cannot be overstated.
View Article and Find Full Text PDFA BtpB-Deleted Brucella Vaccine Strain Provides Dual Advantages: Enhanced Protection and Diagnostic Differentiation Capability in Mice.
Microb Biotechnol
September 2025
College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China.
The Brucella abortus A19 attenuated live vaccine poses potential infection risks during practical applications and interferes with serological diagnostics, thereby affecting quarantine measures and the establishment of disease-free zones. Consequently, this study aimed to reduce its potential virulence, enhance its protective efficacy and differentiate it from wild-type strains by knocking out the immunosuppressive virulence gene btpB in the A19 strain. Using homologous recombination, we successfully obtained the A19ΔBtpB deletion strain.
View Article and Find Full Text PDFAn Indirect Comparison of Diagnostic Accuracy for Seven Different SARS-CoV-2 Serological Assays: A Meta-Analysis and Adjusted Indirect Comparison of Diagnostic Test Accuracy.
Influenza Other Respir Viruses
September 2025
Department of Medical Laboratory, The Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang, China.
Objectives: This study compared the diagnostic accuracy of seven different commercial serological assays for COVID-19, using RT-PCR as the gold standard, through meta-analysis and indirect comparison.
Methods: Fifty-seven studies, published from November 2019 to June 2024, were included. The diagnostic performance of IgA, IgG, and total antibody assays for SARS-CoV-2 was assessed.