Physiological Responses and Transcriptome Analysis of Under Low-Temperature Stress.

species are highly ornamental but sensitive to habitat temperature, making cross-border domestication challenging. In this study, physiological indicators and transcriptome data of 'shizhitou' were analyzed to identify key factors involved in the response to cold. The findings provide a scientific basis for the conservation of germplasm resources and breeding of cold-tolerant varieties. Under prolonged low-temperature stress, significant changes were observed in the physiological indices of 'shizhitou'. Among soluble substances, soluble protein content continuously increased, while soluble sugar content exhibited a fluctuation pattern of increase-decrease-increase. Under prolonged low-temperature stress, significant changes were observed in the physiological indexes of 'shizhitou', while soluble sugar content exhibited a fluctuation pattern of increase-decrease-increase. Overall, soluble sugar and soluble protein contents were significantly positively correlated. Chlorophyll content initially decreased and then increased, whereas peroxidase (POD) and catalase (CAT) activities fluctuated and were negatively correlated with chlorophyll content. Malondialdehyde (MDA) content showed an irregular fluctuation trend. A total of 56,424 unigenes were obtained by transcriptome sequencing, of which 39,278 were annotated, while 10,816 differentially expressed genes (DEGs) were identified, including 5748 up-regulated and 5068 down-regulated genes, with 143 DEGs commonly shared across conditions. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed that low-temperature stress significantly influenced glucose metabolism, lipid metabolism, and amino acid metabolism, and the core pathways of cold stress included zeatin synthesis, hormone signaling, and galactose metabolism. Both physiological responses and transcriptome-based enrichment of DEGs indicate that the redox system and metabolic pathways play crucial regulatory roles in under cold stress.