A new approach to lysozyme in cheese manufacture: boosting key enzyme activities in the ripening process.

J Dairy Res

Facultad de Ingeniería Química, Instituto de Lactología Industrial (CONICET), Santiago del Estero 2829, Santa Fe, Argentina.

Published: November 2024


Category Ranking

98%

Total Visits

921

Avg Visit Duration

2 minutes

Citations

20

Article Abstract

The rational use of lactobacilli and lysozyme (LZ) could be a simple strategy for boosting key enzyme activities during cheese ripening. This study investigated the effect of the application of LZ on the cell envelopes and metabolic potential of two strains of mesophilic lactobacilli: 29 (L29) and 77 (L77). An in vitro study of LZ-sensitivity was carried out on the two strains. In L29 (only), the results of turbidity, culturability and physiological profile as well as fluorescence microscopy images and scanning electron micrographs confirmed a high-level of sensitivity to LZ. Therefore, L29 alone or in combination with LZ as well as a cell-free extract of L29 were used as ripening agents in the manufacture of a mature cheese. The release of peptidases in the cheese matrix by the lysis of L29 was validated and associated with positive effects on the cheese quality, especially the increase of hydrophilic peptide levels.

Download full-text PDF

Source
http://dx.doi.org/10.1017/S0022029925000184DOI Listing

Publication Analysis

Top Keywords

boosting key
8
key enzyme
8
enzyme activities
8
cheese
5
l29
5
approach lysozyme
4
lysozyme cheese
4
cheese manufacture
4
manufacture boosting
4
activities ripening
4

Similar Publications

A series of Cu-based single-atom catalysts (SACs) with asymmetric coordination were designed to accelerate lithium-sulfur (Li-S) chemistry. The electronegativity contrast from the dopant induces a localized electronic asymmetry that amplifies Jahn-Teller distortion at the Cu center. This distortion profoundly modulates the Cu 3d electronic structure and its interaction with Li-S intermediates.

View Article and Find Full Text PDF

Traditional drug discovery methods like high-throughput screening and molecular docking are slow and costly. This study introduces a machine learning framework to predict bioactivity (pIC₅₀) and identify key molecular properties and structural features for targeting Trypanothione reductase (TR), Protein kinase C theta (PKC-θ), and Cannabinoid receptor 1 (CB1) using data from the ChEMBL database. Molecular fingerprints, generated via PaDEL-Descriptor and RDKit, encoded structural features as binary vectors.

View Article and Find Full Text PDF

IL12-based phototherapeutic nanoparticles through remodeling tumor-associated macrophages combined with immunogenic tumor cell death for synergistic cancer immunotherapy.

Biomater Sci

September 2025

Key Laboratory of Biomaterials and Nanotechnology for Cancer Immunotherapy, The Tianjin Key Laboratory of Biomaterials, Institute of Biomedical Engineering, Peking Union Medical College & Chinese Academy of Medical Sciences, Tianjin, 300192, China.

Various cancer therapeutic strategies have been designed for targeting tumor-associated macrophages (TAMs), but TAM reprogramming-based monotherapy is often clinically hindered, likely due to the lack of a coordinated platform to initiate T cell-mediated immunity. Herein, we fabricated reactive oxygen species (ROS)-responsive human serum albumin (HSA)-based nanoparticles (PEG/IL12-IA NPs) consisting of indocyanine green (ICG), arginine (Arg), and interleukin 12 (IL12). Upon laser irradiation, the nanoparticles were found to be able to dissociate, thus facilitating the release of IL12.

View Article and Find Full Text PDF

Creating effective treatments for type 2 diabetes mellitus (T2DM) remains a critical global health challenge. This study investigates the antidiabetic mechanisms of subsp. B-53 ( B-53) in T2DM mice.

View Article and Find Full Text PDF

Unlocking High-Performance Electrochemiluminescence in Supramolecular Coordination Frameworks via π-Bridge Engineering and Aggregation.

Small

September 2025

School of Chemistry and Chemical Engineering, Key Lab of Fuel Cell Technology of Guangdong Province, South China University of Technology, Guangzhou, 510641, China.

Aggregation-induced electrochemiluminescence (AIECL) is a promising strategy for enhancing electrochemiluminescence (ECL) efficiency by minimizing energy loss of excited-state ECL emitters. However, rational design of high-efficiency AIECL emitters is hindered by limited mechanistic understanding and an unclear structure-performance relationship. To address this, four supramolecular coordination frameworks (SCFs) with varying π-bridge structures are synthesized using pyridine-functionalized tetraphenylethene (TPE) as the ligand and Pt(II) as the coordination center.

View Article and Find Full Text PDF