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Article Abstract
Introduction: The human skin, comprising the epidermis, dermis, and subcutaneous fat layers, serves as a critical barrier against external stimuli. The integrity of this barrier function is essential for preventing skin damage and diseases. When compromised, it can lead to various dermatological issues.
Methods: This study investigated the efficacy of J2K55-derived exosomes (LBDEs) on enhancing skin barrier function. High-purity LBDEs were produced and characterized using nanoparticle tracking analysis and Cryo-TEM, concentrated to 1.52×10 particles/mL with sizes ranging from 50 to 200 nm. The LBDEs were then applied to human keratinocytes, HaCaT cells, and a live human skin model to analyze the expression of genes significant to skin barrier function.
Results: In vitro experiments demonstrated that 2.5% LBDEs increased Filaggrin mRNA expression by 301.80% compared to the control. In an ex vivo skin damage model induced by physical stimulation and UVB (Ultraviolet B) irradiation, 1% LBDEs treatment significantly upregulated the expression of key barrier-related proteins, including Aquaporin-3 (180.8%), Claudin-1 (205.4%), Filaggrin (309.9%), Loricrin (365.2%), and Serine palmitoyltransferase (191.3%), in comparison to the friction and UVB-induced control group.
Conclusion: These results suggest that LBDEs have potential in enhancing skin barrier function, as evidenced by increased expression of crucial barrier-related proteins in both in vitro and ex vivo models.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12068317 | PMC |
| http://dx.doi.org/10.2147/CCID.S512793 | DOI Listing |
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