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With socio-economic development, the prevalence of intestinal parasitic diseases has significantly decreased year by year. However, parasitic infections remain a major public health issue globally, particularly in developing countries and regions. Timely diagnosis and treatment are crucial for controlling the spread of these diseases. The traditional Direct Wet Smear Microscopy method, while widely used, is labor-intensive, prone to contamination, and dependent on the skills of the technician. This paper introduces an Automatic Fecal Analyzer, which automates the stool sample processing, offering advantages over the traditional Direct Wet Smear Microscopy method, such as ease of operation, rapid detection, a clean and hygienic working environment, and high sensitivity and specificity, thus enhancing diagnostic efficiency and accuracy. We compared three different methods for fecal analysis: direct wet smear microscopy method, automatic fecal analyzer (AI report), and automatic fecal analyzer (user audit). The AI report uses automated image analysis and machine learning algorithms to identify components like parasites and eggs in fecal samples. This method can process a large number of samples quickly, increasing efficiency. The User Audit also uses an automatic fecal analyzer but includes an additional step of user audit. Experienced technicians review the AI report to enhance the accuracy and reliability of the results.The analyzer demonstrated a sensitivity of 84.31% for AI report and 94.12% for user audits, along with a specificity of 98.71% for AI reports and 99.69% for user audits, making it an invaluable tool for the clinical diagnosis and treatment of parasitic infections.
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http://dx.doi.org/10.3791/67706 | DOI Listing |
BMC Med
September 2025
Department of Microbiota Medicine & Medical Center for Digestive Diseases, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, 210011, Jiangsu, China.
Background: Bile acid malabsorption (BAM) or bile acid diarrhea (BAD) complicates more than 30% of Crohn's disease (CD), yet no non-invasive biomarker reliably identifies patients who will benefit from fecal microbiota transplantation (FMT). We investigated whether serum 7α-hydroxy-4-cholesten-3-one (C4), a hepatic bile-acid synthesis precursor, can predict BAM and FMT response in inflammatory bowel disease (IBD).
Methods: We included 106 pairs of IBD patients treated with FMT from two longitudinal cohorts of prospective trials and 24 matched healthy individuals to identify a multi-omics analysis of microbiota-metabolism and evaluate real-world effectiveness of FMT.
Int J Parasitol
August 2025
Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Via Federico Delpino 1, 80137, Naples, Italy. Electronic address:
Fasciola hepatica and Calicophoron daubneyi are trematodes with significant health and economic impacts on ruminant livestock farms. An effective and reliable diagnosis is essential to control their spread. To improve copromicroscopic diagnosis, the Kubic FLOTAC Microscope (KFM), a portable digital microscope, was designed for both laboratory and field use.
View Article and Find Full Text PDFElife
August 2025
Sagol Department of Neurobiology, Faculty of Natural Sciences, University of Haifa, Haifa, Israel.
In many mammals, including rodents, social interactions are often accompanied by active urination (micturition), which is considered a mechanism for spatial scent marking. Urine and fecal deposits contain a variety of chemosensory signals that convey information about the individual's identity, genetic strain, social rank, and physiological or hormonal state. Furthermore, scent marking has been shown to be influenced by the social context and by the individual's internal state and experience.
View Article and Find Full Text PDFJ Anim Sci
January 2025
Instituto de Ciencia y Tecnología Animal, Universitat Politècnica de València, Valencia 46022, Spain.
In recent years, the production system of Iberian pigs has been significantly intensified, with new technologies playing a crucial role in recording traits such as feed efficiency. These advances also allow the study of different traits related to feeding behavior. In addition, the composition of the gut microbiota has previously been associated with animal health and performance.
View Article and Find Full Text PDFDetecting harmful bacteria in drinking water is a significant concern for public health. Indicator bacteria like E. coli and fecal streptococci serve as markers for fecal contamination of water [Environmental and Pollution Science (2019), pp.
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