Multi-Plug Filtration Purification Combined With Gas Chromatography-Tandem Mass Spectrometry for the Detection of 107 Pesticides in Livestock and Poultry Meat.

Rationale: With the global rise in foodborne diseases, excessive pesticide residues in livestock and poultry meat have emerged as a critical food safety issue. China is a large consumer of livestock and poultry meat, and the traditional national method standard can only detect a few or one type of pesticide in these meats. Establishing a method for the simultaneous detection of multiple types of pesticides in these meats can effectively protect people's health and safety.

Methods: The extraction process was done using acetonitrile direct extraction, followed by clean-up through multi-plug filtration. Separation was achieved using gas chromatography on an HP-5MS capillary column with a programmed temperature gradient, and detection was performed via mass spectrometry in multiple reaction monitoring modes.

Results: The linear ranges of 107 analytes exhibited strong correlation coefficients, all exceeding 0.9901, with limits of quantification spanning from 1.0 to 15.2 μg/kg. The average recoveries, inter-day relative standard deviations (inter-day RSDs), and intra-day relative standard deviations (intra-day RSDs) of the 94 analytes ranged from 70.2% to 120.0%, 4.0% to 16.0%, and 4.5% to 16.8%, respectively, at the three different spiked levels.

Conclusion: A multi-plug filtration purification combined with gas chromatography-tandem mass spectrometry method was successfully applied to the detection of 107 pesticide residues in livestock and poultry meat, which is simple, reliable, and reproducible. It provides a comprehensive solution for the detection of pesticide residues in livestock and poultry meat.