Unveiling the differences between vitexin and isovitexin: From the perspective of sources, green advanced extraction technologies, biological activities, and safety.

Vitexin and isovitexin, as the two of representative flavonoid C-glycosides, are naturally occurring bioactive compounds predominantly found in food plants. Their multiple pharmacological activities have generated a lot of interest in their application in disease management. However, as isomers, vitexin and isovitexin show differences between them in various aspects. To achieve their personalized application in precision nutrition, further research is needed to reveal their differences. Based on the various sources and distribution of vitexin and isovitexin, the advantages and limitations of various green advanced extraction technologies are discussed to improve their purity and activity. Furthermore, vitexin and isovitexin exhibit shared and diverse health benefits from the perspective of in vitro and in vivo research reports. This review contributes to a better understanding of the sustainable production of natural vitexin and isovitexin based on their differences, thus promoting the personalized application of them in the functional foods or pharmaceutical industries.