Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Pig embryonic stem cells were introduced with an MSGN1 upstream region-based reporter system, composed of EGFP sequence under porcine-specific MSGN1 promoter (pMSGN1), through electroporation. The engineered cell line maintained pluripotency similar to that of the parental cell line, and reporter activity was confirmed upon the induction of the paraxial mesoderm. The pMSGN1-EGFP reporter system is a valuable tool for monitoring the differentiation process of the paraxial mesoderm and analyzing induction efficiency, providing insight into developmental mechanisms.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.scr.2025.103724 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Programmable Dual-Phase Electrochemical Biosensor Combines Homogeneous CRISPR/Cas12a Activation with Interfacial Poly-G Signaling for miRNA-21 Detection.
Anal Chem
September 2025
Jiaxing Key Laboratory of Molecular Recognition and Sensing, College of Biological and Chemical Engineering, Jiaxing University, Jiaxing 314001, China.
Despite the promise of electrochemical biosensors in amplified nucleic acid diagnostics, existing high-sensitivity platforms often rely on a multilayer surface assembly and cascade amplification confined to the electrode interface. These stepwise strategies suffer from inefficient enzyme activity, poor mass transport, and inconsistent probe orientation, which compromise the amplification efficiency, reproducibility, and practical applicability. To address these limitations, we report a programmable dual-phase electrochemical biosensing system that decouples amplification from signal transduction.
View Article and Find Full Text PDFCharacterization and Development of Salt-Resistant Aspergillus oryzae for Marine-Derived Enzyme Expression.
Appl Biochem Biotechnol
September 2025
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Marine-derived enzymes often show distinct physiological properties and great potential for industrial use. Salt ions may improve the stability and expression efficiency of marine enzymes, which requires salt-resistant host based expression platform. Aspergillus oryzae of good protein expression and secretion was evaluated and explored for this purpose.
View Article and Find Full Text PDFDownregulation of FOXC1 Modified by METTL3/YTHDF1 Axis Aggravates NLRP3-Mediated Inflammasomes Formation and Cell Pyroptosis in Epilepsy.
FASEB J
September 2025
Department of Hematology, The Second Xiangya Hospital of Central South University, Changsha, Hunan, People's Republic of China.
Epilepsy is a common chronic nervous system disease that threatens human health. However, the role of FOXC1 and its relations with pyroptosis have not been fully studied in epilepsy. Sprague-Dawley rats were obtained for constructing temporal lobe epilepsy (TLE) models.
View Article and Find Full Text PDFA human striatal-midbrain assembloid model of alpha-synuclein propagation.
Brain
September 2025
Okinawa Institute of Science and Technology Graduate University, Okinawa, 904-0495, Japan.
Animal models of the pathology of Parkinson's disease (PD) have provided most of the treatments to date, but the disease is restricted to human patients. In vitro models using human pluripotent stem cells (hPSCs)-derived neural organoids have provided improved access to study PD etiology. This study established a method to generate human striatal-midbrain assembloids (hSMAs) from hPSCs for modeling alpha-synuclein (α-syn) propagation and recapitulating basal ganglia circuits, including nigrostriatal and striatonigral pathways.
View Article and Find Full Text PDFSelf-phosphorylating DNAzyme DK1 enables programmable multi-analyte readout via PfAgo.
Biosens Bioelectron
September 2025
Department of Biological Sciences, Faculty of Science, National University of Singapore, 117543, Singapore; Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 117597, Singapore; Precision Medicine Translational Research Programme (TRP), Yong Loo Lin School
DNAzymes possessing kinase-like activities have long held theoretical promise, yet their practical implementation has remained significantly limited. Notably, DNAzyme kinase 1 (DK1), discovered over two decades ago, exhibits a unique self-phosphorylation capability upon encountering specific substrates like ATP, but its broad-based and programmable applications have not yet been fully realized. In this study, we innovatively couple DK1's autophosphorylation mechanism with the PfAgo to establish a novel programmable cascade sensing platform named RASTEN (Robust pfAgo-based Strategy for POC Testing Non-nucleic Acid and Nucleic Acid).
View Article and Find Full Text PDF