Effect of zein nanoparticles addition on anthocyanin and lutein dual-loaded nanocomposite hydrogels: Structure, physico-chemical and delivery properties.

In this study, lutein-loaded zein nanoparticles (Lu-NPs) were firstly prepared using the antisolvent precipitation method, and the obtained nanoparticles had a spherical shape with an average hydrodynamic diameter of 78.13 nm, a monodispersed distribution and relatively high encapsulation efficiency of 89.11 %. Then, Lu-NPs were added to anthocyanin-loaded arabinoxylan/casein hydrogel (ACN-gel) to fabricate Lu-NPs incorporated nanocomposite hydrogels (Lu-NPs/ACN-gel), which emphasized on the effect of Lu-NPs addition amount (10 %, 20 % and 40 %) on the structure, physico-chemical and delivery properties of nanocomposite hydrogels. It was found that the 10 % or 20 % Lu-NPs addition could not obviously influence the microstructure and gel strength of Lu-NPs/ACN-gel. Meanwhile, hydrophobic and hydrogen bonding interactions were formed between Lu-NPs and the gel matrix. However, with increasing Lu-NPs addition amount to 40 %, the porosity of Lu-NPs/ACN-gel was significantly increased to 48.48 % and the collapsed appearance was observed, which might be due to the formation of Lu-NPs aggregates in the gel network structure. Moreover, 10 % or 20 %Lu-NPs/ACN-gels were found to greatly improve the light and thermal stability of anthocyanin and lutein, and exhibited colon-specific release behavior. These data suggested a possible utilization of Lu-NPs/ACN-gel in gel-related eye care foods.