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Article Abstract
, a fast-growing bacterium, is an emerging chassis of next-generation industrial biotechnology capable of thriving under open and continuous culture conditions. Cadaverine, a valuable industrial C5 platform chemical, has various chemical and biological activities. This study found that exhibited superior tolerance to lysine, the substrate of cadaverine production. For the first time, a cadaverine synthesis pathway was introduced into for whole-cell catalysis of cadaverine from lysine. A high-efficiency cadaverine-producing strain harboring a toxin-antitoxin system, (pSEVA341-pTac--pHbpBC-) with (PN96_RS17440) inactivation, was constructed. In 7 L bioreactors, the cadaverine titer increased from 115 g/L in the original strain to 158 g/L within 11 h of biotransformation, exhibiting a 37% increase in production. Its productivity reached 14.4 g/L/h with a conversion rate as high as 90%. These results confirm as an exceptional chassis for effective cadaverine bioproduction.
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