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Coffee is an economically important crop and one of the most consumed beverages worldwide. Despite the beneficial potential of microbes in coffee fermentation, studies on their application in dry-processed coffee are lacking. Therefore, we evaluated the microbiological, chemical, and sensory changes in Arabica coffee cherries inoculated with Leuconostoc mesenteroides, Saccharomyces cerevisiae (SC), and Aspergillus oryzae during dry processing. Inoculation with these three starter cultures altered the chemical profile of fermented coffee cherries and nearly doubled the 5-caffeoylquinic acid content in the bean compared to the uninoculated group. Additionally, microorganism-inoculated coffee beans showed markedly increased antioxidative capacities. Sensory evaluation revealed improved scores for all inoculated coffees, with SC-inoculated coffee achieving the highest sensory score (84.00 ± 0.25), while the uninoculated group received 80.17 ± 1.04 (p < 0.05). Our results suggest that inoculating specific microorganisms during dry processing can enhance coffee quality by improving the bioactive compound contents and sensory characteristics.
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http://dx.doi.org/10.1016/j.foodchem.2025.143226 | DOI Listing |
Food Chem
August 2025
Beijing Key Laboratory of Flavor Chemistry, Beijing Technology and Business University, Beijing 100048, China. Electronic address:
Yunnan coffee is praised for the sweet caramel aroma and slightly sour taste, but its key flavor compounds and aroma formation mechanisms remain unclear. In this study, the dynamic changes of coffee aroma, amino acids, free fatty acids, free sugars, chlorogenic acids and caffeine at different roasting degrees were investigated by SAFE-GC-MS and HPLC. Roasted coffees exhibited richer flavor profiles, especially caramel, nutty and roasted flavors, while the grassy, cereal and beany flavors of green beans (GB) were significantly diminished.
View Article and Find Full Text PDFPlant Foods Hum Nutr
September 2025
REQUIMTE/LAQV, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, Rua Jorge Viterbo Ferreira, 228, 4050-313, Porto, Portugal.
The growing interest in prebiotic ingredients has led to the valorization of agri-food by-products, such as coffee silverskin, known for its richness in dietary fiber and health-promoting compounds. This study evaluated the impact of in vitro simulation of gastrointestinal digestion on the chemical composition (carbohydrates, caffeine, and chlorogenic acids) and prebiotic potential (probiotic growth, organic acid production, pH, and antioxidant activity) of milled coffee silverskin. The results show stability of polysaccharides during digestion, while caffeine and 5-caffeoylquinic acid were partially released into the bioaccessible fraction.
View Article and Find Full Text PDFInt J Food Sci
August 2025
Department of Pharmaceutical Sciences, University of Piemonte Orientale, Novara, Italy.
This study compared two nontargeted analytical techniques-headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS) and comprehensive two-dimensional gas chromatography-mass spectrometry (GC × GC-MS)-to fingerprint the volatile organic compounds (VOCs) of green beans from Ethiopia, Brazil, Nicaragua, and Guatemala. HS-GC-IMS enabled rapid differentiation of samples, detecting VOC signal regions that effectively clustered samples by origin with minimal preparation. GC × GC-MS offered higher chemical resolution, identifying 98 compounds, including methoxypyrazines, aldehydes, and alcohols, which significantly contributed to interorigin variability.
View Article and Find Full Text PDFFront Genet
August 2025
Sustainable Perennial Crops Laboratory, United States Department of Agriculture, Agriculture Research Service, Beltsville, MD, United States.
Climate change poses significant challenges to global coffee production, particularly for Arabica coffee, which is constrained by a narrow temperature tolerance and a limited genetic pool. This study explores , a species native to West Africa, as a potential alternative to Arabica due to its adaptability to higher temperatures and high-quality flavor profile. Using genome-wide association studies (GWAS), we investigated the genetic basis of phenotypic diversity within accessions from Sierra Leone, focusing on traits related to growth habit, fruit and seed morphology, and plant structural characteristics.
View Article and Find Full Text PDFPharmaceuticals (Basel)
August 2025
Research Group on Bioactive Substances (GISB), Faculty of Pharmaceutical and Food Sciences, University of Antioquia, Medellin 050010, Colombia.
: Most snakebite incidents in Latin America are caused by species of the genus. Their venom induces severe local effects, against which antivenom therapy has limited efficacy. Metabolites derived from have demonstrated anti-inflammatory and anticoagulant properties, suggesting their potential as therapeutic agents to inhibit the local effects induced by venom.
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