Advances in SERS detection method combined with microfluidic technology for bio-analytical applications.

With the advancement of research on life systems and disease mechanisms, the precision of analysis tends to be at a single molecule or single gene level. The surface-enhanced Raman scattering (SERS) method is highly anticipated because of its sensitive detection ability down to a single molecule level. The SERS-based microfluidic platforms retain both advantages of SERS and microfluidics, working in a complementary way. The combination of microfluidics and SERS can provide rapid, non-destructive, high-sensitive, and high-throughput analysis for biological samples, which is of great significance to developing potential biomedical applications, thus occupying an outstanding position among the current research hot topics. This review briefly summarized the recent developments and applications of SERS-based microfluidic platforms in biological analysis. This paper first introduced the SERS-based microfluidic platforms and gave a classification of this method including continuous flow-based method, microarrays-based method, droplet-based method, lateral flow assay (LFA)-based method, and digital-based method. In particular, the bioanalytical applications of SERS-based microfluidic platforms in recent years, including biomolecule detection, cell analysis, and disease diagnosis, have been reviewed. It illustrated that SERS-based microfluidic platforms have great potential in bioanalysis.