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The increased demand for consuming seafood has made seafood production undergo a rapid period of growth. However, seafood has a high risk of contamination from harmful microorganisms and marine toxins which can cause health problems for humans consuming it. Concerning this issue, monitoring seafood safety has become a center of attention for researchers, and developing effective methods for detecting contamination in seafood has become a critical research field. In this context, biosensors have served as a promising approach to monitor seafood contamination. Compared to conventional methods, biosensors have some key benefits such as high sensitivity, selectivity, portability, and user-friendly operation. Along with significant advances in biosensors, processes of seafood monitoring can be simplified and performed outside the laboratory. In this review article, we describe the mechanisms of two main types of biosensors regarding electrochemical and optical biosensors. The current reports within the last five years on the application of these biosensors for seafood monitoring are also summarized.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11677422 | PMC |
http://dx.doi.org/10.3390/mi15121509 | DOI Listing |
Biosensors (Basel)
July 2025
Department of Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Kanagawa, Japan.
Histamine sensing that uses enzymatic reactions is the most common form of testing due to its selectivity for histamine. However, enzymes are difficult to store for long periods of time, and the inactivation of enzymes decreases the reliability of the results. In this study, we developed a novel, quick, and easily operated histamine sensing technique that takes advantage of the histamine redox reaction and does not require enzyme-based processes.
View Article and Find Full Text PDFFood Chem
August 2025
School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo 255049, China; Shandong Province Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo 255049, China; Zibo City Key Laboratory of Agricultur
In this study, a co-reactant-free antifouling electrochemiluminescence (ECL) sensor was prepared based on a macromolecular heterocyclic compound electrocatalytic material and an electroneutral hydrogel. THPP as a macromolecular heterocyclic compound material, can generate reactive oxygen species (ROS). Luminal as an ECL signal source, coupled with THPP to form a THPP-Lum ECL system without co-reactants, replacing the original luminal hydrogen peroxide system, greatly simplifying the construction process of ECL sensors.
View Article and Find Full Text PDFBiosens Bioelectron
November 2025
College of Veterinary Medicine, Institute of Comparative Medicine, Yangzhou University, Yangzhou, 225009, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China; Joint International Research La
Vibrio parahaemolyticus, a significant seafood-borne pathogen, necessitates rapid and precise detection to guarantee food safety. Herein, we developed a visualization assay method by engineering T4 bacteriophage (T4) to display approximately 870 tail spike proteins (TSPs) derived from a lytic bacteriophage specific to target bacteria, along with about 30 gold nanoparticles (GNPs) of 5 nm at distinct capsid domains. Specifically, we fused the small outer capsid protein (Soc) of T4 with a TSP to obtain a fusion protein Soc-VP-TSP.
View Article and Find Full Text PDFTalanta
July 2025
Department of Fundamental Chemistry, Institute of Chemistry, University of São Paulo, Av. Professor Lineu Prestes, 748, 05513-970, São Paulo, SP, Brazil.
Aquatic toxins are potent, naturally occurring compounds produced by dinoflagellates, cyanobacteria, and diatoms during harmful algal blooms (HABs) in both freshwater and marine ecosystems. These toxins can accumulate in seafood, such as scallops, oysters, mussels, and certain fish, posing a significant global threat to human health and the environment. The current methods for detecting these aquatic toxins, such as mouse bioassays, liquid chromatography-tandem mass spectrometry (LC-MS/MS), and high-performance liquid chromatography (HPLC), are limited by time-consuming procedures, high costs, and ethical problems.
View Article and Find Full Text PDFFood Chem X
July 2025
School of Bioengineering and Biosciences, Lovely Professional University, Jalandhar, Punjab, India.
Foodborne diseases pose significant public health and economic challenges worldwide, with conventional pathogen detection methods, such as culture-based assays and PCR, often hindered by the complex food matrix in categories like dairy, seafood, fresh produce, and processed foods. These matrices, containing fats, proteins, biofilms, and salts, interfere with detection accuracy, reducing the sensitivity and robustness of traditional approaches. Nanoparticle-based detection systems have emerged as transformative tools to overcome these challenges, offering enhanced sensitivity, rapid detection, and adaptability to real-time monitoring.
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