Nano-enhanced Fenton/Fenton-like chemistry: integrating peroxidase nanozymes, MOFs, and MXenes for next-generation colorimetric biosensors.

Colorimetric biosensors exhibit promising potential toward molecular analysis with a wide range of sample types such as clinical, environmental, animal, and plant samples due to their high portability, sensitivity, specificity, and accuracy. Colorimetric biosensors rely on chromogenic reactions to transduce biochemical signals into visible color changes. Among the various signal transduction mechanisms, the Fenton/Fenton-like reaction is an outstanding reaction that can be used to detect a broad spectrum of analytes under diverse conditions.

A gold nanoparticle-based colorimetric strategy for DNA detection: principles and novel approaches.

The development of nanotechnology has led to the rapid growth of many different fields, including sensors. Bulky and complex sensor systems are gradually being replaced by streamlined sensor devices with advantages in size, simplicity, cost-effectiveness, and fast response, allowing qualitative detection of target analyte on-site application for clinical diagnosis. Significantly, since the COVID-19 pandemic, research on developing test kits for detecting biological molecules has grown rapidly, with an increasing number of publications.

Biosensors for Seafood Safety Control-A Review.

The increased demand for consuming seafood has made seafood production undergo a rapid period of growth. However, seafood has a high risk of contamination from harmful microorganisms and marine toxins which can cause health problems for humans consuming it. Concerning this issue, monitoring seafood safety has become a center of attention for researchers, and developing effective methods for detecting contamination in seafood has become a critical research field.

Colorimetric polymerase chain reaction mediated by pH indicator: Rapid detection of drug-resistant nosocomial bacteria.

Polymerase chain reaction (PCR) is the gold standard for molecular diagnoses due to its high sensitivity and accuracy. However, conventional PCR exhibits limitations including extended analysis time and complicated sample treatment. To shorten the analysis time of standard three-step PCR, we employed a two-step PCR allowing for fast amplification of nucleic acid in a chip format.

Recent advances in portable devices for environmental monitoring applications.

Environmental pollution remains a major societal problem, leading to serious impacts on living organisms including humans. Human activities such as civilization, urbanization, and industrialization are major causes of pollution. Imposing stricter rules helps control environmental pollutant levels, creating a need for reliable pollutant monitoring in air, water, and soil.

Isothermal amplification-based microfluidic devices for detecting foodborne pathogens: a review.

The gold standard for nucleic acid amplification-based diagnosis is the polymerase chain reaction (PCR). The PCR recognizes the targets such as foodborne pathogens by amplifying their specific genes. The integration of nucleic acid amplification-based assays on microfluidic platforms represents a highly promising solution for convenient, cheap, and effective control of foodborne pathogens.

Microfluidic advances in food safety control.

Food contamination is a global concern, particularly in developing countries. Two main types of food contaminants-chemical and biological-are common problems that threaten human health. Therefore, rapid and accurate detection methods are required to address the threat of food contamination.

Droplet-Based Microfluidics: Applications in Pharmaceuticals.

Droplet-based microfluidics offer great opportunities for applications in various fields, such as diagnostics, food sciences, and drug discovery. A droplet provides an isolated environment for performing a single reaction within a microscale-volume sample, allowing for a fast reaction with a high sensitivity, high throughput, and low risk of cross-contamination. Owing to several remarkable features, droplet-based microfluidic techniques have been intensively studied.

Colorimetric detection of viable antibiotic resistant Enterococcus mediated by cordless operation of reverse transcription loop-mediated isothermal amplification.

In this study, we applied a tube-based reverse transcription loop-mediated isothermal amplification technique using preloaded amplification and detection reagents for simple screening of viable vancomycin-resistant Enterococcus in a cordless manner. We adopted an mRNA-based approach to detect live Enterococcus in vancomycin-treated cultures. We used agarose to preload and store all reagents for amplification and detection inside the tube, which could achieve on-site isothermal nucleic acid amplification and detection in less than 1 h without using sophisticated instruments.

Spinning and Fully Integrated Microdevice for Rapid Screening of Vancomycin-Resistant .

This study introduces a spinning and fully integrated paper-based microdevice that can perform multiple functions, including DNA extraction, amplification, and colorimetric detection, for monitoring two major vancomycin-resistant (VREs), which carry the A and B genes. The spinning microdevice is composed of a stationary part and a spinning part. The square-shaped stationary part has two zones: the lysis and reaction zones.

Nucleic acid amplification-based microfluidic approaches for antimicrobial susceptibility testing.

Because of the global spread of antimicrobials, there is an urgent need to develop rapid and effective tools for antimicrobial susceptibility testing to help clinicians prescribe accurate and appropriate antibiotic doses sooner. The conventional methods for antimicrobial susceptibility testing are usually based on bacterial culture methods, which are time-consuming, complicated, and labor-intensive. Therefore, other approaches are needed to address these issues.

Fully Integrated and Foldable Microdevice Encapsulated with Agarose for Long-Term Storage Potential for Point-of-Care Testing of Multiplex Foodborne Pathogens.

In this study, we fabricated a fully integrated and foldable microdevice encapsulated with 2-hydroxyethyl agarose for long-term storage of reagents for the integration of isothermal amplification and subsequent colorimetric detection for the monitoring of multiplex foodborne pathogens. The microdevice comprises a reaction zone and a detection zone. Both zones were made of a thin polycarbonate film and sealed by an adhesive film to make the microdevice foldable.

Fully integrated and slidable paper-embedded plastic microdevice for point-of-care testing of multiple foodborne pathogens.

This study presents a slidable paper-embedded plastic microdevice fully integrated with DNA extraction, loop-mediated isothermal amplification (LAMP), and colorimetric detection functionalities. The developed microdevice consists of three layers that allow a sliding movement to mix the sample and reagents for DNA purification, amplification, and detection in a sequential manner. An FTA card was employed in the main chamber for DNA extraction and purification from intact bacterial cells.

A foldable isothermal amplification microdevice for fuchsin-based colorimetric detection of multiple foodborne pathogens.

In this study, we have developed a foldable microdevice fully integrating DNA purification, amplification, and detection processes for detecting multiple foodborne pathogens. Specifically, the loop-mediated isothermal amplification (LAMP) technique was combined with a fuchsin-based direct DNA colorimetric detection method. The microdevice was composed of three parts: a sample zone, reaction zone, and detection zone.

A rapid and eco-friendly isothermal amplification microdevice for multiplex detection of foodborne pathogens.

In this study, a plastic microdevice based on loop-mediated isothermal amplification (LAMP) was fabricated for the amplification and on-chip fluorescence detection of multiple pathogens. Papers infused with LAMP reagents and specific primers were embedded inside the multiple reaction chambers of the microdevice. A solution containing the target pathogens was injected into the sample chamber, located in the center of the microdevice, and evenly distributed to the reaction chambers simultaneously via centrifugal force.