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The aim of the trial was to evaluate the influence of diet supplementation with Saccharomyces cerevisiae on nutrient digestibility and milk and mozzarella cheese yield in buffalo cows during the transition period. Twenty buffalo cows in the last month of pregnancy were equally divided into two groups (control, C, and treated, T) homogeneous for parity and milk yield at the previous lactation. The diet of group T was supplemented with 100 g/head/day of a product containing Saccharomyces cerevisiae (Sc 47- CNCM I-4407) for 4 weeks before and after calving. Dry matter intake and the body condition score were not affected by the dietary treatment. In contrast, organic matter, protein, NDF, and ADF digestibility significantly ( < 0.01) increased. Group T showed a higher milk yield (kg 10.5 vs. 9.2, < 0.05) but lower milk fat (g/kg 76.0 vs. 80.1, < 0.05). The buffalo standard milk (FPCM = 740 kcal) yield was higher in the treated group (kg 16.8 vs. 15.0, < 0.05), whereas the mozzarella cheese yield was not affected by the treatment ( > 0.05). These findings indicate that supplementation of the diet of buffaloes with Saccharomyces cerevisiae during the transition period results in notable enhancements in nutrient digestibility and milk yield. These outcomes are worthy of further investigation.
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http://dx.doi.org/10.3390/ani14243689 | DOI Listing |
Int Microbiol
September 2025
School of Basic Sciences, Technology and Engineering, National Open and Distance University, Pasto, Nariño, Colombia.
This study investigates the impact of a defined starter culture on the fermentation of cocoa beans and its influence on the production of volatile and non-volatile compounds related to sensory quality. A microbial consortium comprising Saccharomyces cerevisiae, Pichia kudriavzevii, Levilactobacillus brevis, and Acetobacter okinawensis was selected based on their enzymatic activity and acid regulation properties. Fermentation trials showed that the starter culture enhanced the synthesis of key volatile compounds, particularly esters and higher alcohols, such as 2-phenylethanol and 2-phenylethyl acetate, which contribute floral and fruity aromas.
View Article and Find Full Text PDFAdv Biochem Eng Biotechnol
September 2025
Institute of Process Engineering in Life Sciences, Electrobiotechnology, Karlsruhe Institute of Technology, Karlsruhe, Germany.
While bioprocesses using Escherichia coli, Corynebacterium glutamicum, various species of Bacillus, lactic acid bacteria, Clostridia, the yeasts Saccharomyces cerevisiae and Pichia pastoris, fungi such as Aspergillus niger, and Chinese hamster ovary cells are well established, the high level of microbial diversity has not yet been exploited industrially. However, the use of alternative organisms has the potential to significantly expand the process window of bioprocesses. These extensions include the use of alternative substrates (e.
View Article and Find Full Text PDFJ Cell Biol
October 2025
Department of Cell and Developmental Biology, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.
Carboxy-terminal tails (CTTs) of tubulin proteins are sites of regulating microtubule function. We previously conducted a genetic interaction screen and identified Kip3, a kinesin-8 motor, as potentially requiring the β-tubulin CTT (β-CTT) for function. Here we use budding yeast to define how β-CTT promotes Kip3 function and the features of β-CTT that are important for this mechanism.
View Article and Find Full Text PDFElife
September 2025
Graduate School of Life Science, Hokkaido University, Sapporo, Japan.
DNA replication requires recruitment of Cdc45 and GINS into the MCM double hexamer by initiation factors to form an active helicase, the Cdc45-MCM-GINS (CMG) complex, at the replication origins. The initiation factor Sld3 is a central regulator of Cdc45 and GINS recruitment, working with Sld7 together. However, the mechanism through which Sld3 regulates CMG complex formation remains unclear.
View Article and Find Full Text PDFSynth Syst Biotechnol
December 2025
Division of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 zhongshan Road, Dalian, 116023, PR China.
Engineering yeast cell factories is a feasible approach to produce value chemicals from renewable feedstocks. However, during the production process, reprogramming of the internal metabolic pathways of yeast cells and environmental stress always compromises its production performance. Here, we engineered the robust to enhance the production of fatty alcohols by downregulating the expression of target of rapamycin gene and deleting histone deacetylase gene in .
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