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Objectives: The aim of this study was to determine the effect of apical preparation size and preparation taper on smear layer removal using a metallic needle and a new polymer needle (IrriFlex, Produits Dentaires SA "PD," Vevey, Switzerland).
Materials And Methods: One hundred and eight single-rooted teeth with one canal were randomly divided into four groups according to the preparation and irrigation needle used: G1-30, 0.04 and IrriFlex ( = 25); G2-25, 0.06 and IrriFlex ( = 25); G3-30, 0.04 and metallic needle ( = 25); and G4-25, 0.06 and metallic needle ( = 25). All groups received the same final irrigation protocol and sonic activation. Each tooth was sectioned and observed under a scanning electron microscope (SEM).
Statistical Analysis: Data were statistically analyzed by using one-way and two-way analysis of variance on ranks with a significance level at = 0.05.
Results: For all groups, there was significantly higher smear layer in the apical third ( < 0.001) compared with the coronal and middle thirds. The 25, 0.06 preparation demonstrated better cleaning efficiency than the 30, 0.04 preparation throughout the canal when irrigated with a metallic needle; however, there were no significant differences in the middle and apical thirds when IrriFlex was used. There were also no differences of smear layer removal between G1 and G3 and G2 and G4 in the coronal part. In the middle and apical parts, G1 showed better elimination of smear layer compared with G3. There were slight differences in the middle third between G2 and G4, while G2 showed less cleaning efficiency compared with G4 in the apical third ( = 0.022).
Conclusion: All groups showed less smear layer in the middle and coronal thirds of the canal compared with the apical third. The 25, 0.06 preparation was more effective in removing smear layer compared with the 30, 0.04 preparation. IrriFlex improved irrigation in the 30, 0.04 preparation, while its efficacy was less evident in the 25, 0.06 preparation.
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http://dx.doi.org/10.1055/s-0044-1791682 | DOI Listing |
J Dent
September 2025
Department of Restorative Dentistry, Faculty of Dentistry, Malaya University, Kuala Lumpur, Malaysia. Electronic address:
Objectives: to evaluate the effect of smear-layer deproteinization using papain gel and SPRG-adhesive on marginal-gap, anti-demineralization of enamel and dentin after chemical pH cycling and assess acid-base resistance zone (ABRZ) characteristics.
Methods: Cylindrical cavities were prepared cervically in thirty-two extracted premolars. Teeth were divided into two pretreatment groups (n=16); deproteinization with papain enzyme gel (Papacarie Due, Brazil) for 60 second, and no-deproteinization.
J Endod
August 2025
Department of Bioscience Research, College of Dentistry, University of Tennessee Health Science Center, Memphis, TN, USA; Department of Endodontics(,) College of Dentistry, The University of Tennessee Health Science Center, Memphis, Tennessee; Department of Physiology, College of Medicine, Universit
Introduction: To investigate the combined use of laser, nanobubble water (NB-HO), and calcium hydroxide (Ca(OH)) for endodontic disinfection, particularly in regenerative endodontic cases where maximum effectiveness is needed.
Methods: Canal of human teeth were prepared, sterilized and inoculated with Enterococcus faecalis (E. faecalis).
BMC Oral Health
August 2025
Department of Periodontology, Faculty of Dentistry, Gazi University, Ankara, Türkiye.
Background: Root surface biomodification (RSB) enhances tissue attachment by removing the smear layer, facilitating collagen fibril formation, and promoting clot formation and stabilization. This study aimed to evaluate the efficacy of injectable platelet-rich fibrin (i-PRF), an autologous blood product, as a potential adjunct to ethylenediaminetetraacetic acid (EDTA) for RSB in gingival fibroblast attachment and proliferation in vitro.
Methods: Dentin discs (4 mm in diameter) underwent root surface debridement to remove damaged cementum.
Shanghai Kou Qiang Yi Xue
June 2025
Department of Stomatology, Chifeng University Affiliated Hospital. Chifeng 024000, China. E-mail:
Purpose: To evaluate the effects of Er:YAG laser combined with NaClO on the removal of root canal taint layer, calcium hydroxide clearance and dentin microhardness.
Methods: A total of 318 single-rooted extracted teeth collected from March 2021 to January 2024 were selected, the 13 mm root was taken and mechanically prepared and randomly divided into three groups, with 106 teeth in each group. The control group was rinsed with NaClO, the experimental group 1 was rinsed with NaClO combined with ultrasonic washing, and the experimental group 2 was treated with Er:YAG laser on the basis of NaClO.
J Conserv Dent Endod
August 2025
Department of Orthodontics and Dentofacial Orthopedics, Bharati Vidyapeeth (Deemed to be University) Dental College and Hospital, Pune, India.
Aim: The aim of the study was to evaluate the antimicrobial properties of calcium hydroxide (CH)-loaded polylactic-co-glycolic acid (PLGA) nanoparticles (NPs) and CH when used as an intracanal medicament (ICM).
Methods: Adhering guidelines of the Preferred Reporting Items for Systematic Reviews and Meta-analysis, the review was registered in the Prospective Registration of Systematic Review-CRD42023454265. Databases from January 2000 to December 2023 were examined for studies evaluating the various properties of CH-loaded PLGA NPs compared to conventional ICM-CH.