Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Many attempts have been made to simplify tissue fixation and processing prior to hematoxylin and eosin (H&E) staining, immunohistochemistry (IHC) and genomic DNA extraction. The acetone-methyl benzoate-xylene (AMeX) method, is gaining popularity as an alternative to aqueous formaldehyde fixation followed by alcohol dehydration. We have assessed the efficacy of AMeX for the quality of extracted genomic DNA in addition to H&E and IHC in biopsy specimens of human oral mucosal lesions of size not more than 1 cm. H&E staining showed similar results when AMeX method was compared with conventional fixation and processing. Improved IHC staining quality was observed in the sections of AMeX-processed tissue, as were improved quality and quantity of genomic DNA.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1080/10520295.2024.2434743 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The renaissance of multipurpose AMeX method over conventional tissue processing method in the current diagnostic era: a comparative study.
Biotech Histochem
November 2024
Department of Oral & Maxillofacial Pathology and Microbiology, Maulana Azad Institute of Dental Sciences, New Delhi, India.
Article Synopsis
- Researchers are exploring simpler methods for tissue fixation and processing before staining and DNA extraction, with a focus on the AMeX method as a promising alternative to traditional techniques.
- The AMeX method was tested on human oral mucosal biopsy specimens to assess its effectiveness in H&E staining, immunohistochemistry, and DNA quality.
- Results indicated that AMeX provided comparable H&E staining results, while enhancing the quality of immunohistochemistry and yielding better genomic DNA quality and quantity.
Nivolumab plus ipilimumab versus sunitinib for first-line treatment of advanced renal cell carcinoma: extended 8-year follow-up results of efficacy and safety from the phase III CheckMate 214 trial.
Ann Oncol
November 2024
Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, USA.
Background: Nivolumab plus ipilimumab (NIVO+IPI) has demonstrated superior overall survival (OS) and durable response benefits versus sunitinib (SUN) with long-term follow-up in patients with advanced renal cell carcinoma (aRCC). We report updated analyses with 8 years of median follow-up from CheckMate 214.
Patients And Methods: Patients with aRCC (N = 1096) were randomized to NIVO 3 mg/kg plus IPI 1 mg/kg Q3W × four doses, followed by NIVO (3 mg/kg or 240 mg Q2W or 480 mg Q4W); or SUN (50 mg) once daily for 4 weeks on, 2 weeks off.
Treatment-free survival and partitioned survival analysis of patients with advanced renal cell carcinoma treated with nivolumab plus ipilimumab versus sunitinib: 5-year update of CheckMate 214.
J Immunother Cancer
July 2024
Harvard Medical School, Boston, Massachussetts, USA.
Article Synopsis
- Immunotherapy, like the combination of nivolumab and ipilimumab (NIVO+IPI), can lead to prolonged disease control and treatment-free survival (TFS) for cancer patients, even after stopping treatment, which is not accounted for by standard survival measures.
- A study analyzed data from 1,096 advanced renal cell carcinoma patients to estimate TFS, comparing those treated with NIVO+IPI against sunitinib (SUN), focusing on both survival time and treatment-related adverse events (TRAEs).
- Results showed that 48% of patients on NIVO+IPI were alive after 5 years, with a significant difference in mean TFS compared to SUN, particularly in favorable-risk patients, highlighting the importance of considering
Efficacy of morning versus afternoon aerobic exercise training on reducing metabolic syndrome components: A randomized controlled trial.
J Physiol
December 2024
Exercise Physiology Lab at Toledo, University of Castilla-La Mancha, Toledo, Spain.
A supervised intense aerobic exercise program improves the health of individuals with metabolic syndrome (MetS). However, it is unclear whether the timing of training within the 24 h day would influence those health benefits. The present study aimed to determine the influence of morning vs.
View Article and Find Full Text PDFNeddylation inhibition sensitises renal medullary carcinoma tumours to platinum chemotherapy.
Clin Transl Med
May 2023
Center for Precision Environmental Health, Baylor College of Medicine, Houston, Texas, USA.
Background: Renal medullary carcinoma (RMC) is a highly aggressive cancer in need of new therapeutic strategies. The neddylation pathway can protect cells from DNA damage induced by the platinum-based chemotherapy used in RMC. We investigated if neddylation inhibition with pevonedistat will synergistically enhance antitumour effects of platinum-based chemotherapy in RMC.
View Article and Find Full Text PDF