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Article Abstract
Amino acid residues are crucial to protein structure and function and have links to various human diseases. Here, we present a protocol for screening functional lysine residues across the human genome. We describe steps for designing lysine codon-targeting single-guide RNAs (sgRNAs), constructing an sgRNA library, conducting cell fitness screenings, and acquiring screening results. This approach leverages base editing and high-throughput screening techniques to systematically examine functional amino acid residues. For complete details on the use and execution of this protocol, please refer to Bao et al..
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11550167 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2024.103418 | DOI Listing |
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