Ru@UiO-66-NH MOFs-Based Dual Emission Ratiometric Fluorescence for Sensitive Sensing of Arginine.

Biosensors (Basel)

State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

Published: October 2024


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Article Abstract

Arginine has been widely applied in the food industry as coloring agents, flavoring agents, and nutritional fortifiers. It is also one of the major components of feed additives. Currently, methods for the highly selective detection of arginine remain absent. For accurate and sensitive detection of L-arginine, a novel ratiometric fluorescence assay based on Ru@UiO-66-NH was developed and demonstrated in this study. Under optimized detection conditions, the limit of detection (LOD) of this assay for L-arginine was 2.32 μM, which is superior to most assays reported to date. Meanwhile, Ru@UiO-66-NH showed good stability within 30 days, demonstrating the wide applicability of the proposed assay. The spike-and-recovery rates of the proposed assay for L-arginine in real samples (e.g., tea, grape juice, and serum) were 84.27-113.09%. Overall, the proposed assay showed high sensitivity, good reproducibility, and excellent stability in the detection of L-arginine in both buffer and real samples.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11505678PMC
http://dx.doi.org/10.3390/bios14100512DOI Listing

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