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Article Abstract
Catalytic DNA circuits, serving as signal amplification strategies, can enable simple and accurate detection of pathogenic bacteria in complex matrices but suffer from low reaction rates and depths. Herein, we design an enzyme-accelerated catalytic hairpin assembly (EACHA) in which duplex DNA products are converted into hairpin reactants to continue participating in the next circuit reaction with the assistance of RNase H. Profiting from the high recyclability of the reactants, EACHA exhibits an approximately 37.6-fold enhancement in the rate constant and a two-order-of-magnitude improvement in sensitivity compared to conventional catalytic hairpin assembly (CHA). By integrating an allosteric probe with EACHA, a one-pot method is developed for rapid and direct detection of S. enterica Enteritidis (S. Enteritidis). This method is capable of detecting 15 CFU mL of S. Enteritidis within 20 min, which is superior to that of real-time PCR. By testing 60 milk samples, we demonstrate this method's high accuracy in discriminating contaminated samples, with an area under the curve (AUC) of 0.997. Moreover, this method can be employed to accurately diagnose early-stage infected mice, with an AUC of 1.00 for feces samples and 0.986 for serum samples. Therefore, this study offers a simple and feasible method for identifying pathogens in complex matrices.
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Corrigendum to "Enzyme-accelerated catalytic DNA circuits enable rapid and one-pot detection of bacterial pathogens" [Biosens. Bioelectron. 267 (2025) 116822].
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School of Medicine, Xi'an Jiaotong University, Xi'an, 710061, PR China; Department of Clinical Laboratory, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710061, PR China. Electronic address:
Enzyme-accelerated catalytic DNA circuits enable rapid and one-pot detection of bacterial pathogens.
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School of Medicine, Xi'an Jiaotong University, 710061, Xi'an, PR China; Department of Clinical Laboratory, The First Affiliated Hospital of Xi'an Jiaotong University, 710061, Xi'an, PR China. Electronic address:
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- Catalytic DNA circuits are useful for detecting harmful bacteria, but they have limitations in speed and effectiveness, prompting the development of EACHA, which enhances reaction rates and sensitivity.
- EACHA improves detection capabilities significantly, achieving about 37.6 times faster rates and allowing detection of S. enterica Enteritidis at very low concentrations (15 CFU/mL) in just 20 minutes.
- The method has shown high accuracy in testing milk samples (AUC of 0.997) and diagnosing early infections in mice, proving its potential for identifying pathogens in complex environments.
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