Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Introduction: Diabetic macular edema (DME) is a major cause of vision loss in the sick with diabetic retinopathy. The occurrence of DME is closely related to the breakdown of neurovascular coupling; however, its underlying mechanism has not been fully elucidated. The aim of this study was to investigate the diagnostic biomarkers and potential molecular mechanisms associated with neurovascular coupling in DME.
Methods: The differential expression analysis, STEM, and WGCNA were performed from GSE160306 to identify hub genes. The gene expression was validated by RT-qPCR. The relevant mechanisms of action were investigated through GO, KEGG, and GSEA analyses, as well as co-expression networks. Additionally, the LASSO regression analysis and a nomogram were used to demonstrate the diagnostic effectiveness of the model. Finally, the GenDoma platform was utilized to identify drugs with potential therapeutic effects on DME.
Results: Neurotrophic factor receptor (NGFR) was identified as a hub gene related to neurovascular coupling and DME. The expression of NGFR was verified by RT-qPCR in cells. GSEA analysis indicated that high expression of NGFR may affect immunity and inflammatory pathway, thereby regulating neurovascular coupling and mediating the development of DME. The NGFR co-expression network was constructed, which exhibited the correlation with the neurotrophin signaling pathway. Moreover, a diagnostic model for DME based on NGFR and PREX1 demonstrated relatively good diagnostic performance using LASSO regression analysis and the nomogram. And then the GenDoma platform identified drugs with potential therapeutic effects on DME.
Conclusion: The high expression of NGFR may lead to abnormal neurovascular coupling and participate in the occurrence of DME by regulating the immunity, inflammatory and neurotrophin signaling pathway. Detection of NGFR and related expression genes may be beneficial for monitoring the occurrence and development of DME.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11427358 | PMC |
| http://dx.doi.org/10.3389/fmolb.2024.1332842 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Mechanistic Insights and Translational Therapeutics of Neurovascular Unit Dysregulation in Vascular Cognitive Impairment.
J Integr Neurosci
August 2025
Key Laboratory of Modern Toxicology of Ministry of Education; School of Basic Medical Sciences, Nanjing Medical University, 211166 Nanjing, Jiangsu, China.
Cognitive impairment represents a progressive neurodegenerative condition with severity ranging from mild cognitive impairment (MCI) to dementia and exerts significant burdens on both individuals and healthcare systems. Vascular cognitive impairment (VCI) represents a heterogeneous clinical continuum, spanning a spectrum from subcortical ischemic VCI (featuring small vessel disease, white matter lesions, and lacunar infarcts) to mixed dementia, where vascular and Alzheimer's-type pathologies coexist. While traditionally linked to macro- and microvascular dysfunction, the mechanisms underlying VCI remain complex.
View Article and Find Full Text PDFDiverse and distributed haemodynamic effects of theta burst stimulation in the prefrontal cortex.
Neuroimage Rep
September 2025
School of Psychology, Faculty of Medicine and Health, University of Leeds, LS2 9JT, UK.
Background: Theta Burst Stimulation (TBS) is a form of non-invasive brain stimulation that can induce neuroplastic changes in the underlying intracortical areas. It has significant potential in clinical and research settings for modulating cognitive and motor performance. Little is known about how TBS affects oxygenations levels within and across brain hemispheres during stimulation of the Dorsolateral Prefrontal Cortex (DLPFC).
View Article and Find Full Text PDFSNAKE: A modular realistic fMRI data simulator from the space-time domain to k-space and back.
Imaging Neurosci (Camb)
September 2025
CEA, Joliot, NeuroSpin, Université Paris-Saclay, Gif-sur-Yvette, France.
We propose a new, modular, open-source, Python-based 3D+time realistic functional magnetic resonance imaging (fMRI) data simulation software. SNAKE or imulator from eurovascular coupling to cquisition of -space data for xploration of fMRI acquisition techniques. It is the first simulator to simulate the entire chain of fMRI data acquisition, from the spatio-temporal design of evoked brain responses to various 3D sampling strategies of k-space data with multiple coils.
View Article and Find Full Text PDFCrossing Pathological Boundaries: Multi-Target Restoration of the Neurovascular Unit in Alzheimer's and Vascular Dementia-From Modern Therapeutics to Traditional Chinese Medicine.
Aging Dis
September 2025
Key Laboratory of Basic Theory Research on Traditional Chinese Medicine, Harbin, 150040, China.
Alzheimer's disease (AD) and vascular dementia (VD) are the two most common forms of dementia, and they share common mechanisms, especially in regard to neurovascular dysfunction. There has been increasing evidence that the disruption of the neurovascular unit (NVU), which consists of endothelial cells, pericytes, astrocytes, microglia, neurons, and basement membrane, is one of the key early events in both AD and VD. The objective of this review is to summarize the structure and physiological function of the NVU, then discuss the pathological remodeling of the NVU in AD and VD and finally, show emerging evidence of multi-target approaches that restore the NVU and neurovascular protection.
View Article and Find Full Text PDF7 Tesla fMRI characterisation of the cortical-depth-dependent BOLD response in early human development.
bioRxiv
August 2025
Early Life Imaging Research Department, School of Biomedical Engineering and Imaging Sciences, King's College London, London, UK.
Human cortical development leading up to and around birth is crucial for lifelong brain function. Cortical activity can be studied using BOLD fMRI, however, previously limited sensitivity and spatial specificity has constrained understanding of how its emergence relates to functional cortical circuitry and neurovascular development at the mesoscale. To resolve this, we used ultra-high-field 7 Tesla MRI to acquire submillimetre resolution BOLD-fMRI data from 40 newborns and 4 adults.
View Article and Find Full Text PDF