Molecular study of the KCNJ11 gene and its correlation with Prakriti to preventing and managing type 2 diabetes.

In Ayurveda, every individual is believed to possess a unique entity known as which distinguishes them from others physically, physiologically, and psychologically. This entity also determines an individual's response to a particular stimulus, and it is believed that such responses are not solely determined by genetics. The present research aims to validate the Ayurvedic concept of from a modern molecular perspective to strengthen the personalized and precise treatment approach. A study was conducted to investigate the role of the KCNJ11gene in the susceptibility of individuals to type 2 diabetes mellitus (T2DM) with their metabolic status. The research involved allele mining on three major groups - , and in 112 patients with T2DM and 112 healthy individuals. The KCNJ11 gene, responsible for insulin secretion membrane pore formation, was analyzed to determine the susceptibility of different types to T2DM. The MutPred tool predicted the molecular cause of disease-related amino acid substitution. According to the study, only and were diagnosed with diabetes, while all three types were present in the control group of healthy individuals. A protein model was prepared, and the changes resulting from mutations were observed for each group in their protein sequence, both as synonymous and non-synonymous mutations. Ultimately, these changes contributed to the manifestation of T2DM. Based on the findings, it appears that groups may experience changes in protein function due to nonsynonymous mutations and differences in amino acids at the protein level.