98%
921
2 minutes
20
Chromatographic analysis of phenolic phytochemicals in foods has significantly advanced over the past decade (2014-2024), meeting increasing demands for precision and efficiency. This review covers both conventional and advanced chromatographic techniques used for detecting phenolic phytochemicals in foods. Conventional methods like High-Performance Liquid Chromatography, Ultra High-Performance Liquid Chromatography, Thin-Layer Chromatography, and Gas Chromatography are discussed, along with their benefits and limitations. Advanced techniques, including Hydrophilic Interaction Liquid Chromatography, Nano-LC, Multidimensional Liquid Chromatography, and Capillary Electrophoresis, are highlighted for their innovations and improved capabilities. The review addresses challenges in current chromatographic methods, emphasizing the need for standardized and validated procedures according to the Food and Drug Administration, European Cooperation for Accreditation of Laboratories, and The International Organization for Standardization guidelines to ensure reliable and reproducible results. It also considers novel strategies for reducing the environmental impact of chromatographic methods, advocating for sustainable practices in analytical chemistry.
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11276055 | PMC |
http://dx.doi.org/10.3390/foods13142268 | DOI Listing |
Biomed Chromatogr
October 2025
College of Food Science and Pharmaceutical Engineering, Zaozhuang University, Zaozhuang, China.
To evaluate the quality of pomegranate peels from different cultivars, pomegranate peel samples from 47 cultivars were compared and classified based on fingerprints and chemical components obtained using HPLC-PDA-MS/MS combined with chemometric methods. Three pattern recognition methods, namely, hierarchical cluster analysis, principal component analysis, and partial least square-discriminant analysis, were used to establish classification models. Results showed that the contents of 10 components from pomegranate peel were determined.
View Article and Find Full Text PDFBrain Behav
September 2025
School of Physical Education and Health, Henan University of Chinese Medicine, Zhengzhou, China.
Background: Clinical and basic research suggests that exercise is a safe behavioral intervention and effective in improving cognition in vascular dementia (VD). However, despite global efforts, there is still no effective method to completely cure VD. This study aimed to investigate the effects of long-term exercise pretreatment on typical VD pathology in a rat model, and further compare the neuroprotective impacts of different exercise modalities on VD rats.
View Article and Find Full Text PDFCerebrovasc Dis Extra
August 2025
Background: This study investigates the impact of trimethylamine oxide (TMAO) on recurrent cerebral infarction in minor ischemic stroke (MIS).
Methods: A rat model was used, with dietary choline levels adjusted to vary TMAO levels. TMAO was quantified via liquid chromatography-mass spectrometry (LC-MS), and histological changes in brain and aortic tissues were analyzed using HE staining.
Rapid Commun Mass Spectrom
December 2025
Department of Pharmacy, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.
Rationale: Chrysotoxine, a bibenzyl derivative from the stems of Dendrobium medicinal herbs, has recently emerged as a promising therapeutic candidate for cervical cancer. This study aimed to characterize chrysotoxine metabolites across multiple hepatocyte species and in rat urine.
Methods: Metabolites were identified and characterized using liquid chromatography coupled with benchtop Orbitrap high-resolution mass spectrometry (LC-Orbitrap-MS/MS) combined with Compound Discoverer software.
J Sep Sci
September 2025
Departament de Química Analítica, Facultat de Química, MINTOTA Research Group, Universitat De València, Burjassot, Spain.
Spirulina is considered a superfood due to its chlorophylls. Two new methods for the determination of chlorophylls and β-carotene were developed here, one based on in-tube solid-phase microextraction (IT-SPME) coupled online to nanoliquid chromatography (nanoLC) with diode array detection (DAD), and the other on ultraviolet-visible diffuse reflectance spectroscopy (UV-vis DRS). A protocol to extract the pigments from spirulina was proposed using ethanol (1.
View Article and Find Full Text PDF