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In digital holographic microscopy (DHM), achieving large field of view (FOV) imaging while maintaining high resolution is critical for quantitative phase measurements of biological cell tissues and micro-nano structures. We present a quantitative phase image stitching guided by reconstructed intensity images in one-shot double FOV multiplexed DHM. Double FOVs are recorded simultaneously through frequency division multiplexing; intensity feature pairs are accurately extracted by multi-algorithm fusion; aberrations and non-common baselines are effectively corrected by preprocessing. Experimental results show that even if phase images have coherent noise, complex aberrations, low overlap rate and large size, this method can achieve high-quality phase stitching.
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http://dx.doi.org/10.1364/BOE.523051 | DOI Listing |
Anal Methods
September 2025
Jilin Province Product Quality Supervision and Inspection Institute, Changchun 130103, China.
A method for determination of ten kinds of sweeteners in soybean products by multi-plug filtration cleanup (-PFC) combined with ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was established. The sample was extracted with acetonitrile (containing 1% formic acid), degreased by using -hexane liquid-liquid extraction and purified by solid phase extraction using an -PFC column (Oasis PRiME HLB). The analytes were separated by using a Waters ACQUITY UPLC® BEH C (2.
View Article and Find Full Text PDFNurs Res
September 2025
College of Nursing & Institute of Nursing Research, Korea University, Seoul, South Korea.
Background: Existing research fails to address the complex nature of nonspecific chronic lower back pain (cLBP ) despite its detrimental effect on economic, societal, and medical expenditures.
Objectives: We developed a nurse-led, mobile-delivered self-management intervention-Problem-Solving Pain to Enhance Living Well (PROPEL-M)-and evaluated its usability, feasibility, and initial efficacy for South Korean adults with nonspecific cLBP.
Methods: This study was composed of two phases: (a) lab and field usability testing for a gamified mobile device application; and (b) a pilot study employing a one-arm pre-test and post-test design among adults aged 18-60 years with nonspecific cLBP.
Eur J Immunol
September 2025
Department of Quantitative Biomedicine, University of Zurich, Zurich, Switzerland.
Memory T cells, a sizable compartment of the mature immune system, enable enhanced responses upon re-infection with the same pathogen. We have recently shown that virus-experienced innate acting T (T) cells can modulate infectious or autoimmune diseases through TCR-independent IFN-γ production. However, how these cells arise remains unclear.
View Article and Find Full Text PDFTurkiye Parazitol Derg
September 2025
Ege University Faculty of Medicine, Department of Parasitology, İzmir, Türkiye.
Objective: Leishmaniasis, caused by protozoan parasites of the spp., presents significant global health challenges, with visceral leishmaniasis (VL) and cutaneous leishmaniasis forms causing severe morbidity and mortality. Macrophages serve as primary host cells, where spp.
View Article and Find Full Text PDFAim: To explore the factors affecting the sustainable improvement of nurses' evidence-based practice (EBP) competency after receiving an EBP training program.
Design: A sequential mixed-methods study.
Methods: Thirty-seven ICU nurses participated from an adult ICU in Egypt.