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The current concepts emphasize the fundamental role of reactive oxygen species (ROS) as signaling molecules that coordinate defense mechanisms, cell death, and the growth and development processes in plants. However, due to the inherent reactivity of ROS, achieving precise control over their levels within plant cells, both spatially and temporally, becomes important to effectively harness the potential of ROS signaling while concurrently minimizing the risk of oxidative damage. Ascorbate is an exceptional antioxidant and contributes to the antioxidant defense system in plants. Its role is further reinforced by the presence of ascorbate peroxidases and enzymes responsible for recycling ascorbate from its oxidized forms. Ascorbate metabolism plays a pivotal role in averting oxidative damage and facilitates meticulous regulation of ROS signal availability. This chapter outlines the preferred protocol for the measurement of ascorbate.
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http://dx.doi.org/10.1007/978-1-0716-3826-2_8 | DOI Listing |
J Ethnopharmacol
September 2025
Institute of Materia Medica, Jinzhou Medical University, Jinzhou, Liaoning 121001, China. Electronic address:
Ethnopharmacology Relevance: Tangningtongluo Tablets (TNTL), a novel Miao ethnic medicine for treating type 2 diabetes mellitus (T2DM) and its complications. However, its potential bioactive components and the pharmacological mechanisms underlying its therapeutic effects remain unclear.
Aim Of The Study: This study aims to preliminarily explore the protective effects of TNTL and its active components on pancreatic cells via the PI3K/Akt/FoxO1 pathway and further investigate the underlying mechanisms.
Bioorg Chem
September 2025
Key Innovation Laboratory for Deep and Intensive Processing of Yanbian High Quality Beef (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Yanbian University, Yanji 133002, PR China; Department of Food Science and Technology, College of Agricultural, Yanbian Univ
In this study, bovine peptide‑calcium chelates (BBP-Ca) were prepared via enzymatic hydrolysis to generate peptides and fermentation to obtain soluble calcium ions, which were then chelated together. The structural characteristics of BBP-Ca were comprehensively analyzed using FTIR, SEM, and UV spectroscopy. Additionally, its antioxidant capacity was evaluated by examining its protective effects against oxidative stress-induced damage in Caco-2 cells.
View Article and Find Full Text PDFEcotoxicol Environ Saf
September 2025
Department of Veterinary Medicine, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, Guangdong 524088, China; South China Branch of National Saline-Alkali Tolerant Rice Technology Innovation Center Zhanjiang, Guangdong 524088, China. Electronic address:
Aflatoxin B1 (AFB1)-induced hepatotoxicity is a common toxic disease in poultry farming. However, there is currently a lack of effective pharmaceutical interventions for treating AFB1. Astaxanthin (AST), a natural carotenoid, exhibits potent antioxidant and immune-enhancing properties.
View Article and Find Full Text PDFVet Microbiol
September 2025
Engineering Research Center of Southwest Animal Disease Prevention and Control Technology for Ministry of Education of the People's Republic of China, International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Key Laboratory of Animal Disease and Human Health
Duck plague is a highly contagious disease caused by duck plague virus (DPV) infection, leading to high morbidity (up to 100 %) and mortality rates (up to 95 %) among ducks. Mitochondria are essential organelles for virus replication. It is crucial to deepen the understanding of mitochondrial homeostasis and the interaction between mitochondrial proteins after viral infection.
View Article and Find Full Text PDFACS Nano
September 2025
Department of Emergency and Critical Care Medicine, The Fourth Affiliated Hospital of Soochow University, Suzhou 215124, China.
Acute lung injury (ALI) is characterized by the excessive accumulation of reactive oxygen species (ROS), which triggers a severe inflammatory cascade and the destruction of the alveolar-capillary barrier, leading to respiratory failure and life-threatening outcomes. Considering the limitations and adverse effects associated with current therapeutic interventions, developing effective and safe strategies that target the complex pathophysiological mechanisms of ALI is crucial for improving patient outcomes. Herein, we developed an inhalable, multifunctional nanotherapeutic (MSCNVs@CAT) by encapsulating catalase (CAT) in mesenchymal-stem-cell-derived nanovesicles (MSCNVs).
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