Metabolism of L-threonate, an ascorbate degradation product, requires a protein with L-threonate metabolizing domains in Arabidopsis.

L-Threonate is one of the major degradation products of ascorbate in plants. While bacteria can utilize L-threonate as a sole carbon source by converting it to dihydroxyacetone phosphate, a glycolysis intermediate, through a three- or four-step metabolic pathway, the corresponding processes in plants remain uncharacterized. Remarkably, an Arabidopsis gene encodes a unique protein containing domains homologous to all three enzymes involved in the bacterial three-step pathway.

Dark-induced decrease in ascorbate levels in Arabidopsis leaves occurs independently of ascorbate peroxidase and oxidase, recycling enzymes, and senescence signaling.

Ascorbate is a key antioxidant that protects plant cells from oxidative damage. While plants actively synthesize ascorbate during the day, its degradation becomes prominent under prolonged dark conditions. Since ascorbate degradation begins with its oxidized form, dehydroascorbate (DHA), this process inherently requires ascorbate oxidation.

Feasibility of a nurse-initiated brief cognitive behavioral strategy intervention program for symptom clusters experienced by patients with advanced non-small cell lung cancer.

Objective: To assess the feasibility of a nurse-initiated brief cognitive behavioral strategy (CBS) intervention program targeting pain and fatigue symptoms among the pain and fatigue/anorexia symptom clusters experienced by patients with advanced non-small cell lung cancer (NSCLC).

Methods: In this single-group, pre-post test study, 15 NSCLC outpatients undergoing medical treatment participated. After providing informed consent, participants completed a baseline questionnaire and received a booklet detailing brief cognitive-behavioral techniques (e.

Measurements of Ascorbate and Dehydroascorbate in Plants Using High-Performance Liquid Chromatography.

The current concepts emphasize the fundamental role of reactive oxygen species (ROS) as signaling molecules that coordinate defense mechanisms, cell death, and the growth and development processes in plants. However, due to the inherent reactivity of ROS, achieving precise control over their levels within plant cells, both spatially and temporally, becomes important to effectively harness the potential of ROS signaling while concurrently minimizing the risk of oxidative damage. Ascorbate is an exceptional antioxidant and contributes to the antioxidant defense system in plants.

[The Hokkaido Medical Personnel Training Plan Connecting Humans and Medicine].

The Hokkaido medical personnel training plan connecting humans and medicine aims to train"Medical personnel for genome medicine"," Medical personnel for rare cancer and childhood cancer", and"Medical personnel who promote cancer measures according to patient's life stage". We have worked on preparing medical professionals who undergo training courses not only in the graduate school but also in the community medicine centers cooperating with central medical centers in Hokkaido. Furthermore, we have been committed to training medical staff who provide comprehensive healthcare for patients with cancer cross-regionally, cross-sectionally, and tumor-agnostically and researchers who can pursue genome medicine.

Impact of Symptom Clusters on Quality of Life Outcomes in Patients from Japan with Advanced Nonsmall Cell Lung Cancers.

Objective: Identify symptom clusters based on symptoms experienced by patients with advanced nonsmall cell lung cancers (NSCLCs), and examine the relationship between the symptom clusters and impairment in everyday life and quality of life (QOL).

Methods: Using the M.D.