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Many hosts utilize the ubiquitin system to defend against viral infection. As a key subunit of the ubiquitin system, the role of polyubiquitin in the viral infection of insects is unclear. Here, we identified the full-length cDNA of the polyubiquitin-C (UBC) gene in , the small brown planthopper (SBPH). was expressed in various tissues and was highly expressed in salivary glands, midgut, and reproductive systems. Furthermore, the expression profiles in the developmental stages showed that was ubiquitously expressed in seven developmental stages and was highest expressed in female adults with SBPH. qRT-PCR analyses indicated that rice stripe virus (RSV) infection promoted the expression. Knockdown of mRNA via RNA interference increased RSV accumulation. These findings suggest that LsUBC inhibits RSV accumulation in .
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http://dx.doi.org/10.3390/insects15030149 | DOI Listing |
Front Plant Sci
August 2025
Rice Science Center, Kasetsart University, Nakhon Pathom, Thailand.
Introduction: Rice is mainly consumed by half of the world's population. The imminent climate change and population growth expected in the next 30 years will outpace the current rice production capacity, posing risks to food and nutrition security in developing nations. One simplified approach to address this challenge is to improve photosynthetic capacity by increasing chlorophyll content in leaves and stems.
View Article and Find Full Text PDFPlant Cell Environ
September 2025
State Key Laboratory of Agricultural and Forestry Biosecurity, Institute of Plant Virology, Vector-Borne Virus Research Center, Fujian Agriculture and Forestry University, Fuzhou, China.
Rice viral diseases pose severe threats to global food security, with over 20 viruses identified in China alone. The advent of high-throughput sequencing has accelerated the discovery of novel viruses in cultivated and wild rice, unveiling previously undetected threats. This review systematically summarises newly discovered rice viruses over the past decade, analyzing their genomic characteristics, transmission modes, and pathogenic mechanisms.
View Article and Find Full Text PDFData Brief
October 2025
Department of Computer Science and Engineering, Daffodil International University, Dhaka, Bangladesh.
This manuscript presents a comprehensive, expert-annotated dataset comprising 19,000 rice leaf images, including 2,753 original images and 16,247 augmented images, sourced from the Bangladesh Rice Research Institute (BRRI). The dataset includes seven disease classes: Healthy (603 original images), Rice Blast (696 original images), Scald (421 original images), Leaf-folder Injury (247 original images), Insect Infestation (281 original images), Rice Stripes (266 original images), and Tungro Disease (239 original images). These images, captured under varying environmental conditions using smartphone cameras, accurately reflect real-world conditions.
View Article and Find Full Text PDFViruses
August 2025
Department of Plant Medicals, Gyeongkuk National University, Andong 36729, Republic of Korea.
Insects and their bacterial endosymbionts form intricate ecological relationships, yet their role in host-pathogen interactions are not fully elucidated. The small brown planthopper (), a polyphagous pest of cereal crops, acts as a key vector for rice stripe virus (RSV), a significant threat to rice production. This study aimed to compare the endosymbiont community structures of nonviruliferous and RSV-viruliferous populations using 16S rRNA gene sequencing with high-throughput sequencing technology.
View Article and Find Full Text PDFSci Adv
August 2025
Guangdong Basic Research Center of Excellence for Precise Breeding of Future Crops, South China Agricultural University, Guangzhou 510642, China.
RNA viruses often remodel host intracellular membranes to establish specialized replication compartments through viral protein-induced phase separation. However, the mechanisms underlying membrane remodeling and the characteristics that render these sites conducive to replication remain poorly understood, particularly in plant negative-strand RNA viruses. Here, we demonstrate that the phosphoprotein (P) of rice stripe mosaic virus (RSMV) forms biomolecular condensates via liquid-liquid phase separation (LLPS) to recruit essential components for viral replication factories (VFs).
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