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Article Abstract
Background: N6-methyladenosine (mA) is the most frequently occurring interior modification in eukaryotic messenger RNA (mRNA), and abnormal mRNA modifications can affect many biological processes. However, mA's effect on the metabolism of antiplatelet drugs for the prevention of ischemic stroke (IS) remains largely unclear.
Methods: We analyzed the mA enzymes and mA methylation in peripheral blood samples of IS patients with/without clopidogrel resistance (CR), and the peripheral blood and liver of rat models with/without CR. We also compared the effect of mA methylation on the expression of the drug-metabolizing enzymes (CYP2C19 and CYP2C6v1) in CR and non-CR samples.
Results: Methyltransferase-like 3 (METTL3), an mA enzyme, was highly expressed in the peripheral blood of patients with CR, and in both the peripheral blood and liver of rats with CR. This enzyme targets CYP2C19 or CYP2C6v1 mRNA through mA methylation, resulting in low expression of CYP2C19 or CYP2C6v1 mRNA. Consequently, this leads to decreased clopidogrel metabolism and CR.
Conclusion: The METTL3-mediated methylation of CYP2C19 mRNA may aggravate CR in IS patients.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10921439 | PMC |
| http://dx.doi.org/10.1515/med-2024-0899 | DOI Listing |
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