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Capturing fine spatial, spectral, and temporal information of the scene is highly desirable in many applications. However, recording data of such high dimensionality requires significant transmission bandwidth. Current computational imaging methods can partially address this challenge but are still limited in reducing input data throughput. In this paper, we report a video-rate hyperspectral imager based on a single-pixel photodetector which can achieve high-throughput hyperspectral video recording at a low bandwidth. We leverage the insight that 4-dimensional (4D) hyperspectral videos are considerably more compressible than 2D grayscale images. We propose a joint spatial-spectral capturing scheme encoding the scene into highly compressed measurements and obtaining temporal correlation at the same time. Furthermore, we propose a reconstruction method relying on a signal sparsity model in 4D space and a deep learning reconstruction approach greatly accelerating reconstruction. We demonstrate reconstruction of 128 × 128 hyperspectral images with 64 spectral bands at more than 4 frames per second offering a 900× data throughput compared to conventional imaging, which we believe is a first-of-its kind of a single-pixel-based hyperspectral imager.
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http://dx.doi.org/10.1038/s41467-024-45856-1 | DOI Listing |
J Vis Exp
August 2025
Laser Biomedical Research Center, G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology.
We present multimodal confocal Raman micro-spectroscopy (RS) and tomographic phase microscopy (TPM) for quick morpho-chemical phenotyping of human breast cancer cells (MDA-MB-231). Leveraging the non-perturbative nature of these advanced microscopy techniques, we captured detailed morpho-molecular data from living, label-free cells in their native physiological environment. Human bias-free data processing pipelines were developed to analyze hyperspectral Raman images (spanning Raman modes from 600 cm to 1800 cm, which uniquely characterize a wide range of molecular bonds and subcellular structures), as well as morphological data from three-dimensional refractive index tomograms (providing measurements of cell volume, surface area, footprint, and sphericity at nanometer resolution, alongside dry mass and density).
View Article and Find Full Text PDFSci Rep
July 2025
Innovation Center Computer Assisted Surgery (ICCAS), Faculty of Medicine, Leipzig University, 04103, Leipzig, Germany.
Hyperspectral imaging (HSI) shows significant promise in the medical field for tissue detection and perfusion assessment. To extend its application to intraoperative diagnosis, laparoscopic cameras combining a high resolution color video and simultaneous HSI were developed. Spatial scanning in these cameras is performed through a push-broom motor driving a line-scan spectrograph.
View Article and Find Full Text PDFIEEE Trans Image Process
January 2025
Hyperspectral video (HSV) offers valuable spatial, spectral, and temporal information simultaneously, making it highly suitable for handling challenges such as background clutter and visual similarity in object tracking. However, existing methods primarily focus on band regrouping and rely on RGB trackers for feature extraction, resulting in limited exploration of spectral information and difficulties in achieving complementary representations of object features. In this paper, a spatial-spectral fusion network with spectral angle awareness (SSF-Net) is proposed for hyperspectral (HS) object tracking.
View Article and Find Full Text PDFSci Data
May 2025
Research Center on Software Technologies and Multimedia Systems, Universidad Politécnica de Madrid (UPM), Madrid, 28031, Spain.
Hyperspectral imaging (HSI) and machine learning (ML) have been employed in the medical field for classifying highly infiltrative brain tumours. Although existing HSI databases of in vivo human brains are available, they present two main deficiencies. First, the amount of labelled data are scarce, and second, 3D-tissue information is unavailable.
View Article and Find Full Text PDFIEEE Trans Image Process
January 2025
Hyperspectral video (HSV) provides rich spectral-spatial-temporal information, enabling the capture of complex object dynamics beyond the limitations of conventional single- and multi-modal tracking. However, current HSV tracking methods face challenges such as data scarcity, band gaps, spectral fragmentation, temporal underutilization, and high computational load, which constrain performance. In this article, we present SpectralTrack, a novel HSV tracking framework with spectral-spatial fusion and memory enhancement.
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