98%
921
2 minutes
20
Olfactory-mediated behaviors in fish are often examined in artificial microcosms that enable well-controlled treatments but fail to replicate environmental and social contexts. However, observing these behaviors in nature poses challenges. Here, we describe a protocol for recording sea lamprey (Petromyzon marinus) behaviors in a natural system. We describe steps for administering and verifying accurate odorant concentrations, surveying sea lamprey abundance, and tracking sea lamprey movements. We also detail procedures to analyze treatment effects on pheromone-mediated spawning in a high-density population. For complete details on the use and execution of this protocol, please refer to Scott et al..
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10876582 | PMC |
http://dx.doi.org/10.1016/j.xpro.2024.102891 | DOI Listing |
Conventional dietary assessments are challenging in hematophagous species, particularly in sea lamprey (). However, recent technological developments and molecular approaches have provided an attractive alternative through the use of DNA metabarcoding. While DNA metabarcoding has been used for dietary analyses in numerous species, including lampreys, applications of universal primers that detect a diverse set of prey items can be limited by the amplification of predator DNA.
View Article and Find Full Text PDFGlycobiology
August 2025
Department of Surgery, Beth Israel Deaconess Medical Center, National Center for Functional Glycomics, Harvard Medical School, Boston, MA.
A major challenge in the glycosciences is the scarcity of sensitive and specific glycan-binding reagents, such as monoclonal antibodies, for detecting and isolating glycans. Here we report the development and characterization of new monoclonal antibodies (mAbs) that bind carbohydrate-based red blood cell (RBC) antigens including the ABO(H) antigens. This approach exploits the immune system of the sea lamprey (Petromyzon marinus), which strongly responds to human glycans to enable the generation of high affinity antibodies.
View Article and Find Full Text PDFbioRxiv
July 2025
Graduate Institute of Biomedical Sciences, Chang Gung University, Tao-Yuan City, Taiwan.
Inflammation is a hallmark of immune responses. Its mechanistic underpinnings in mammals are well-defined: pro-inflammatory cytokines of the interleukin 1 (IL-1) superfamily establish and support microenvironments that promote immune cell activities. Despite a growing number of reports on inflammatory processes and components of the IL-1 signaling axis in several invertebrate lineages, orthologs of these central cytokines have not been detected outside of the jawed vertebrates.
View Article and Find Full Text PDFAnimals (Basel)
July 2025
Department of Neurosciences, Faculty of Medicine, University of Montreal, Montreal, QC H3T 1J4, Canada.
Lampreys are primitive fish that rely significantly on olfactory cues throughout their complex life cycle. The olfactory system of the sea lamprey () is among the best characterized in vertebrates. In recent decades, tremendous advances have been made by isolating individual compounds from sea lampreys that can replicate natural behavior when artificially applied in the wild.
View Article and Find Full Text PDFIntegr Environ Assess Manag
July 2025
Department of Biology, Michigan Technological University, Houghton, MI, USA.
Dam removals and fish passage can enhance aquatic connectivity but may also promote upstream transport of legacy contaminants by migratory fish. This study assessed the potential for contaminant biotransport in Michigan's Boardman River following the planned removal of the Union Street Dam and installation of FishPass, a selective fish passage facility. We quantified polychlorinated biphenyls (PCBs), mercury (Hg), and organochlorine pesticides in carcass and egg samples from migratory species including Chinook and coho salmon, migratory rainbow trout, common white and longnose sucker, lake trout, walleye, and sea lamprey.
View Article and Find Full Text PDF