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The effects of fermentation on barley starch were studied using dy-1. Changes in multi-scale structure and physicochemical properties of barley starch were studied. The chain structure results revealed that fermentation could increase the content of short chain and medium short chain by breaking down long amylopectin side chains in barley and increase amylose content by debranching amylopectin. Also, fermentation promoted the arrangement of short chains into short order structure, leading to the enhancement of hydrogen bond interaction. Furthermore, it improved the helical structure content and relative crystallinity of barley starch by degrading the amorphous structure of barley starch. In terms of physicochemical properties, fermentation inhibited the hydration characteristics of barley starch, thus improving its thermal stability. It also enhanced shear stability, resistance to short-term aging and digestion, and improved gel texture properties. These findings offer potential for the processing and nutritional regulation of fermented barley products.
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http://dx.doi.org/10.1039/d3fo04395a | DOI Listing |
Carbohydr Polym
November 2025
Department of Plant and Environmental Sciences, University of Copenhagen, DK-1871 Frederiksberg C, Denmark; Department of Food Science and Nutrition, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China. Electronic address:
This study investigates the phenomenon that, in contrast to amylopectin-rich starch granules, high amylose starch (HAS) granules typically exhibit high hydrolytic resistance manifested as low density of enzyme attack sites on the starch granule surface. However, among the various types of examined HAS granules, we identified differences in enzymatic resistance. We associated this effect as a result of variations in specific rate of the enzymatic reaction, with intermediate affinity leading to the highest enzymatic efficacy characteristic for the Sabatier principle.
View Article and Find Full Text PDFJ Anim Sci
August 2025
Department of Animal Biosciences, University of Guelph, Guelph, ON, Canada.
Metabolic availability (MA) of amino acids (AA) in feedstuffs has been assessed in pigs and humans using the indicator amino acid oxidation (IAAO) method. Our lab previously reported higher methionine (Met) content but lower MA in chicken meal (ChM) as compared to peas. However, the MA of met in peas or ChM were not quantified due to the absence of a crystalline AA reference diet, assumed to have 100% MA.
View Article and Find Full Text PDFFoods
August 2025
Key Laboratory of Geriatric Nutrition and Health, Beijing Technology and Business University, Ministry of Education, Beijing 100048, China.
This study modified corn, oat, barley, and buckwheat starches using a Henan-specific sourdough starter, revealing that the initial starch architecture governs differentiated functional transformations. Pore-dominant starches (corn/buckwheat) underwent "inside-out" enzymatic pathways-corn starch exhibited a 38.21% reduced particle size through pore expansion, with long amylopectin chain degradation forming thermally stable gels, establishing it as an ideal base for anti-staling sauces and frozen dough.
View Article and Find Full Text PDFFood Chem
August 2025
School of Chemical Sciences, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand. Electronic address:
Barley is primarily used in animal feed and malt production, and the structure-property relationship of barley starch (BS) remains understudied. This study examined the physicochemical and structural properties of BS from 25 genotypes and explored the structure-property relationship in the model system. For the first time, the structures and properties of BSs of unimodal and bimodal size distributions were systematically compared.
View Article and Find Full Text PDFPlant Biotechnol J
August 2025
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China.
Starch content and composition are key factors determining yield and quality of barley, but the molecular mechanisms regulating barley starch synthesis remain unclear. In this study, we identified an APETALA2/Ethylene-Responsive factor (AP2/ERF) family gene HvAP2-12, which has typical transcription factor characteristics. Overexpression of HvAP2-12 had no effect in total starch and amylose content, whereas the gene-edited mutant lines showed increased total starch and significantly decreased amylose content.
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