Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Background: Mesenchymal stem cells (MSCs) have protective effects on the cornea, lacrimal gland, retina, and photoreceptor cell damage, which may be mediated by exosomes (exos) released by MSCs.
Aim: To investigate the ameliorating effect of exos derived from different MSCs on retinal ganglion cell (RGC) injury induced by hydrostatic pressure.
Methods: The RGC injury model was constructed by RGC damage under different hydrostatic pressures (40, 80, 120 mmHg). Then RGCs were cultured with adipose-derived stem cell (ADSC)-Exos and bone marrow-derived stem cell (BMSC)-Exos. Cell Counting Kit-8, transmission electron microscopy, flow cytometry, immunofluorescence, real-time quantitative polymerase chain reaction, and western blotting were performed to detect the ameliorating effect of exos on pressure-induced RGC injury.
Results: ADSC-Exos and BMSC-Exos were successfully isolated and obtained. The gibbosity of RGCs was lower, the cells were irregularly ellipsoidal under pressure, and the addition of ADSC-Exos and BMSC-Exos significantly restored RGC morphology. Furthermore, the proliferative activity of RGCs was increased and the apoptosis of RGCs was inhibited. Moreover, the levels of lactate dehydrogenase and apoptosis-related proteins were increased, and the concentrations of antiapoptotic proteins and neurotrophic factors were decreased in damaged RGCs. However, the above indicators were significantly improved after ADSC-Exos and BMSC-Exos treatment.
Conclusion: These findings indicated that ADSC-Exos and BMSC-Exos could ameliorate RGC injury caused by hydrostatic pressure by inhibiting apoptosis and increasing the secretion of neurotrophic factors.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10762527 | PMC |
| http://dx.doi.org/10.4252/wjsc.v15.i12.1077 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Recombinant human collagen hydrogels with different stem cell-derived exosomes encapsulation for wound treatment.
J Nanobiotechnology
March 2025
Department of Rheumatology and Immunology, Nanjing Drum Tower Hospital, School of Medicine, Southeast University, Nanjing, China.
Exosomes-loaded hydrogels have potential value in wound treatment. Current studies focus on improving hydrogels' biocompatibility and optimizing different stem cell-derived exosomes for better therapeutic effect. Herein, we present a novel biocompatible recombinant human collagen (RHC) hydrogel loading with different MSCs-derived exosomes for promoting wound healing.
View Article and Find Full Text PDFStem cell-derived exosomes for ischemic stroke: a conventional and network meta-analysis based on animal models.
Front Pharmacol
October 2024
The First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou, China.
Article Synopsis
- The study evaluated the effectiveness of stem cell-derived exosomes in treating ischemic stroke and examined the best ways to administer them.
- A comprehensive review of research found that different administration routes (intravenous, intranasal, intracerebral) varied in success, with intravenous being the most effective in reducing stroke-related damage.
- Specific types of exosomes, particularly those from adipose and bone marrow stem cells, showed significant benefits in reducing stroke damage, while engineered exosomes performed better than their unmodified counterparts.
Effect of Exosomes From Bone Marrow-Derived Mesenchymal Stromal Cells and Adipose-Derived Stromal Cells on Bone-Tendon Healing in a Murine Rotator Cuff Injury Model.
Orthop J Sports Med
January 2024
Department of Sports Medicine, Xiangya Hospital, Central South University, Changsha, China.
Background: Bone-tendon injury is characterized by poor self-healing. It is established that exosomes are favorable for tissue repair and regeneration. However, their effect on bone-tendon healing has not yet been determined.
View Article and Find Full Text PDFADSC-Exos outperform BMSC-Exos in alleviating hydrostatic pressure-induced injury to retinal ganglion cells by upregulating nerve growth factors.
World J Stem Cells
December 2023
Department of Ophthalmology, Affiliated Hospital of Yunnan University/Yunnan Eye Hospital, Kunming 650021, Yunnan Province, China.
Background: Mesenchymal stem cells (MSCs) have protective effects on the cornea, lacrimal gland, retina, and photoreceptor cell damage, which may be mediated by exosomes (exos) released by MSCs.
Aim: To investigate the ameliorating effect of exos derived from different MSCs on retinal ganglion cell (RGC) injury induced by hydrostatic pressure.
Methods: The RGC injury model was constructed by RGC damage under different hydrostatic pressures (40, 80, 120 mmHg).
Extracellular vesicle-loaded hydrogels for tissue repair and regeneration.
Mater Today Bio
February 2023
Department of Plastic and Aesthetic (Burn) Surgery, The Second Xiangya Hospital, Central South University, Changsha, 410011, People's Republic of China.
Extracellular vesicles (EVs) are a collective term for nanoscale or microscale vesicles secreted by cells that play important biological roles. Mesenchymal stem cells are a class of cells with the potential for self-healing and multidirectional differentiation. In recent years, numerous studies have shown that EVs, especially those secreted by mesenchymal stem cells, can promote the repair and regeneration of various tissues and, thus, have significant potential in regenerative medicine.
View Article and Find Full Text PDF