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A paper-based ratiometric fluorescent sensing platform has been developed for glucose detection based on a dual-emission fluorescent probe consisting of carbon quantum dots (C QDs) and CdTe QDs. When the two kinds of QDs are mixed, the fluorescence of C QDs is reversibly quenched by CdTe QDs. However, in the presence of glucose, the fluorescence of CdTe QDs is quenched by HO catalyzed by glucose oxidase (GOx), which restores the fluorescence of C QDs. The proposed paper-based ratiometric fluorescent sensing platform exhibited good sensitivity and selectivity towards glucose. The working linear range was 0.1 mM to 50 mM with a limit of detection (LOD) of 0.026 mM. Additionally, the proposed paper-based sensor possesses viability for the determination of glucose in actual urine samples.
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http://dx.doi.org/10.1039/d3ra07082d | DOI Listing |
J Fluoresc
September 2025
School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, 541004, China.
The pervasive concern regarding veterinary drug residues in food necessitates advanced detection solutions, particularly addressing limitations of conventional methods reliant on large-scale instrumentation that incur prolonged analysis duration, complex sample preparation, and lack of real-time on-site capability. A portable "single response-on" molecularly imprinted ratiometric fluorescent paper-based sensor was developed for quantifying fleroxacin (FLX) residues in animal-derived foods, wherein B, N-co-doped MXene quantum dot (B, N-MQD) was synthesized and combined with BCP-Eu as dual-emission fluorophores, while FLX- molecularly imprinted polymer (FLX-MIP) was engineered using functionalized Nano-SiO as the carrier. Concentration-dependent fluorescence enhancement at 574 nm was exhibited with invariant reference signal at 411 nm, achieving a 36-fold lower detection limit (0.
View Article and Find Full Text PDFAdv Healthc Mater
September 2025
Hubei Engineering Technology Research Center of Spectrum and Imaging Instrument, Electronic Information School, Wuhan University, Wuhan, 430072, China.
Accurate and rapid detection of urinary creatinine (CR) is critical for early kidney disease screening and efficient healthcare resource management. In this study, a novel colorimetric-fluorescent sensor is developed by integrating nitrogen-doped carbon dots (N-CDs) and copper nanoclusters (CuNCs) with gold nanoparticles (AuNPs), leveraging fluorescence resonance energy transfer (FRET) to enhance sensitivity and selectivity. The sensor functions within a detection range of 1-50 mm, with peak responsiveness at 17 mm, utilizing paper-based substrates for a low-cost and portable application.
View Article and Find Full Text PDFMicrochem J
November 2024
Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, Nebraska 68198-6858, USA.
, a versatile human pathogen, significantly impacts global health causing a broad spectrum of medical conditions that range from minor skin infections to life-threatening diseases. The clinical importance of is underscored by its resistance to multiple antibiotics and formation of biofilms, providing protection against antimicrobials and immune responses. To date, the identification of antimicrobial-resistant (AMR) strains, such as methicillin-resistant (MRSA) and vancomycin-intermediate (VISA), requires time-consuming and expensive methodologies, including culture-based, molecular, and phenotypic techniques.
View Article and Find Full Text PDFFood Chem
November 2025
Xinjiang Key Laboratory of New Energy and Energy Storage Technology, Xinjiang Institute of Technology, Aksu 843100, China. Electronic address:
A smartphone-assisted fluorescent and colorimetric detection method for nitrite based on 2,3-diaminonaphthalene (DAN) and gold nanoclusters (AuNCs) paper strip was developed. DAN reacts with NO via a coupling reaction under acidic conditions to generate naphthotriazole (NAT), emitting strong blue fluorescence at 435 nm. The red fluorescence of AuNCs at 650 nm is quenched by the reduction of nitrite.
View Article and Find Full Text PDFTalanta
November 2025
Department of Biology and Chemistry, Texas A&M International University, Laredo, TX, 78041, United States. Electronic address:
This review explores the evolution and application of fluorescence sensors based on quantum dots (QDs) for detecting environmental and biological analytes across diverse real-world scenarios and complex sample matrices and also categorizes different types of quantum dots, such as carbon dots (C-dots), graphene quantum dots (GQDs), and metal-doped QDs and examines their properties, including tunable fluorescence, low toxicity, and photostability, which make them ideal for a variety of applications. Key sensing mechanisms, including Förster Resonance Energy Transfer (FRET) and fluorescence quenching, are discussed alongside innovations like paper-based, ratiometric, and turn-on/turn-off sensors. Additionally, case studies are provided to showcase the application of these sensors in environmental and biomedical fields, where they provide rapid, sensitive, and cost-effective solutions.
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