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Ultrasound has been widely used as a green and efficient non-thermal processing technique to assist with enzymatic hydrolysis. Compared with traditional enzymatic hydrolysis, ultrasonic-pretreatment-assisted enzymatic hydrolysis can significantly improve the efficiency of enzymatic hydrolysis and enhance the biological activity of substrates. At present, this technology is mainly used for the extraction of bioactive substances and the degradation of biological macromolecules. This review is focused on the mechanism of enzymatic hydrolysis assisted by ultrasonic pretreatment, including the effects of ultrasonic pretreatment on the enzyme structure, substrate structure, enzymatic hydrolysis kinetics, and thermodynamics and the effects of the ultrasonic conditions on the enzymatic hydrolysis results. The development status of ultrasonic devices and the application of ultrasonic-assisted enzymatic hydrolysis in the food industry are briefly described in this study. In the future, more attention should be paid to research on ultrasound-assisted enzymatic hydrolysis devices to promote the expansion of production and improve production efficiency.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10647539 | PMC |
http://dx.doi.org/10.3390/foods12214027 | DOI Listing |
Annu Rev Microbiol
September 2025
3Department of Biology, Faculty of Philosophy, Sciences and Letters at Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
Plant biomass has emerged as a cornerstone of the global bioenergy landscape because of its abundance and cost-effectiveness. The cell wall of plant biomass is an intricate network of cellulose, hemicellulose, and lignin. The hydrolysis of cellulose and hemicellulose by holoenzymes converts these polymers into monosaccharides and paves the way for the production of bioethanol and other bio-based products.
View Article and Find Full Text PDFLangmuir
September 2025
State Key Laboratory of Synthetic Biology, School of Synthetic Biology and Biomanufacturing, Frontiers Science Center for Synthetic Biology (MOE), and Key Laboratory of Systems Bioengineering (MOE), Tianjin University, Tianjin 300350, China.
Effective degradation and detoxification of the highly toxic organophosphate pesticide methyl parathion (MP) are important for pollution treatment and sustainable development. Enzymatic hydrolysis of MP by organophosphate hydrolase (OPH) is an effective way. However, hydrolytic product 4-nitrophenol (4-NP) remains environmentally hazardous.
View Article and Find Full Text PDFAnesth Analg
September 2025
From the Department of Anesthesiology.
Background: Total knee arthroplasty (TKA) is a surgical procedure that induces intense acute postoperative pain, but the mechanisms that amplify post-TKA pain remain incompletely understood. Endocannabinoids, such as 2-arachidonoylglycerol (2-AG), are endogenous lipids that can produce antinociceptive effects. However, hydrolysis of 2-AG by monoacylglycerol lipase (MAGL) generates arachidonic acid, the precursor to a host of eicosanoids that enhance pain.
View Article and Find Full Text PDFJ Sci Food Agric
September 2025
State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, China.
Background: Understanding starch behavior under various processing conditions is important for the development of novel food products with tailored nutritional profiles. This study investigated changes to the structure and properties of native corn starch (NCS) and biomimetic starch-entrapped microspheres following thermal and enzymatic treatments.
Results: Heat-treated microspheres showed more birefringence and structural order than native starch, indicating incomplete gelatinization due to the alginate matrix.
Food Res Int
November 2025
Department of Food Materials and Process Design Engineering, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran; Institute of Leisure Agriculture, Shandong Academy of Agricultural Sciences, Jinan 250100, China. Electronic address:
Hen eggs are rich in proteins, which are a potential source of bioactive peptides. Incubation of fertilized egg changes the egg protein, which may affect the properties and activity of derived peptides. To understand these metamorphoses, hydrolysate fractions of 10-day incubated chicken embryo (CE) proteins of 0.
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