Article Synopsis
- Single-cell transcriptomic studies identified 125 neocortical cell types from postmortem samples, and this research involved 75 adult patients undergoing epilepsy and tumor surgeries.
- Significant individual differences were observed in the abundance and gene expression of certain cell types, especially deep-layer glutamatergic neurons and microglia.
- The study found that factors like age, sex, ancestry, and disease state explained only a small part of the observed variability, with genomic variations linked to the expression of 150 to 250 genes across most cell types.
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Article Abstract
Single-cell transcriptomic studies have identified a conserved set of neocortical cell types from small postmortem cohorts. We extended these efforts by assessing cell type variation across 75 adult individuals undergoing epilepsy and tumor surgeries. Nearly all nuclei map to one of 125 robust cell types identified in the middle temporal gyrus. However, we found interindividual variance in abundances and gene expression signatures, particularly in deep-layer glutamatergic neurons and microglia. A minority of donor variance is explainable by age, sex, ancestry, disease state, and cell state. Genomic variation was associated with expression of 150 to 250 genes for most cell types. This characterization of cellular variation provides a baseline for cell typing in health and disease.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11702338 | PMC |
| http://dx.doi.org/10.1126/science.adf2359 | DOI Listing |
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