Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Phagosome acidification and proteolysis are essential processes in the immune response to contain and eliminate pathogens. In recent years, there has been an increased desire for a rapid and accurate method of assessing these processes in real-time. Here, we outline the development of a multiplexed assay that allows simultaneous monitoring of phagosome acidification and proteolysis in the same sample using silica beads conjugated to pHrodo and DQ BSA. We describe in detail how to prepare the bi-functional particles and show proof of concept using differentially activated macrophages. This multiplexed spectrophotometric assay allows rapid and accurate assessment of phagosome acidification and proteolysis in real-time and could provide valuable information for understanding the immune response to pathogen invasion.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10456865 | PMC |
| http://dx.doi.org/10.3389/fimmu.2023.1204223 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
ESAT-6 of Mycobacterium tuberculosis downregulates cofilin1, leads to filamentous actin enrichment and reduces the phagosome acidification in infected macrophages, which are partially reversed by a single methionine mutation.
Tuberculosis (Edinb)
August 2025
Mycobacteria Research, Bacterial and Parasite Disease Biology, Rajiv Gandhi Centre for Biotechnology, Thycaud P.O., Trivandrum, 695014, Kerala, India. Electronic address:
Mycobacterium tuberculosis (M. tuberculosis) persists within macrophages by evading phagosome maturation. In this study, we considered the role of actin dynamics in this process.
View Article and Find Full Text PDFThe hidden dancers in water: the symbiotic mystery of and free-living amoebae.
Front Microbiol
August 2025
School of Laboratory Medicine, Jilin Medical University, Jilin, China.
, a Gram-negative bacillus, is the primary etiological agent of Legionnaires' disease, a severe respiratory infection. The symbiotic relationship between and free-living amoebae (FLAs), particularly spp., represents a critical intersection of microbial ecology and human pathogenesis.
View Article and Find Full Text PDFCepharanthine Enhances MHC-I Antigen Presentation and Anti-Tumor Immunity in Melanoma via Autophagy Inhibition.
Cells
August 2025
Research Center of Integrative Medicine, School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
Major histocompatibility complex class I (MHC-I)-mediated antigen presentation plays a pivotal role in anti-tumor immunity by enabling CD8 T cells to recognize and eliminate malignant cells. In melanoma, modulation of this pathway is critical for improving the efficacy of immunotherapies. Our study demonstrates that the natural compound Cepharanthine (CEP) exhibits notable antitumor activity by enhancing MHC-I-mediated antigen presentation.
View Article and Find Full Text PDFProton-activated chloride channel governs phagosome-mediated antibacterial immunity in peritoneal macrophages.
J Exp Med
November 2025
Department of Physiology, Pharmacology and Therapeutics, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
The success of phagosome degradation relies on the ability of phagocytes to regulate the maturation of phagosomes. However, its underlying molecular mechanisms remain poorly understood. Here, we identify the proton-activated chloride (PAC) channel as a key negative regulator of phagosome maturation.
View Article and Find Full Text PDFEnzymological and transcriptomic analyses reveal the insecticidal mechanism of 4-propargyloxybenzenesulfonamide as a V-ATPase inhibitor.
Pestic Biochem Physiol
September 2025
College of Chemistry & Pharmacy, Shaanxi Key Laboratory of Natural Products & Chemical Biology, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China; Key Laboratory for Botanical Pesticide R&D of Shaanxi Province, Yangling, Shaanxi 712100, People's Republic of China. Electr
The vacuolar-type H-ATPase (V-ATPase) has emerged as a promising molecular target for the development of new pesticides. In our previous work, we employed computer-aided drug design (CADD) strategies targeting the V-ATPase H subunit of the Mythimna separata. Through homology modeling, virtual screening, and lead compound synthesis, we successfully identified 4-propargyloxybenzenesulfonamide as a potent insecticidal compound.
View Article and Find Full Text PDF