Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Objective: To investigate the prevalence of a tet(A) gene variant and its role in developing high-level tigecycline resistance among carbapenem-resistant Klebsiella pneumoniae (CRKP) clinical isolates.
Methods: The mechanism of high-level tigecycline resistance in CRKP mediated by a tet(A) variant was explored by induction experiments, antimicrobial susceptibility testing, whole-genome sequencing and bioinformatics analysis. The amplification and overexpression of the tet(A) variant were measured by the determination of sequencing depth, gene copy numbers, and qRT-PCR.
Results: A high rate (62.1%, 998/1607) of tet(A) variant carriage was observed among 1607 CRKP clinical isolates from Henan Province, China. High-level tigecycline resistance could rapidly develop by the amplification of the tet(A) variant in these isolates. The analysis of the raw sequencing data and the plasmid mapping depth revealed that the ΔtnpA homologous sequence of Tn1721 supports the amplification of the region that harbours the tet(A) variant by forming a large number of repeat arrays through translocatable units (TUs). Moreover, the epidemiological analysis of tet(A) variant-carrying structures among 1607 clinical CRKPs showed that the TU structure is widely present.
Conclusion: The presence of a tigecycline resistance-mediating tet(A) variant in CRKP clinical isolates represents a greater health concern than initially thought and should be monitored consistently.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.cmi.2023.07.030 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Detection and Genomic Characteristics of NDM-19- and QnrS11-Producing O101:H5 Strain Phylogroup A: ST167 from a Poultry Farm in Egypt.
Microorganisms
July 2025
Laboratory of Food Microbiology and Hygiene, Graduate School of Integrated Sciences for Life, Hiroshima University, Higashihiroshima 739-8528, Japan.
This study describes the first complete genomic sequence of an NDM-19 and QnrS11-producing multidrug-resistant (MDR) isolate collected from a fecal swab from a poultry farm in 2019 in Egypt. The was identified by PCR screening and DNA sequencing. The isolate was then subjected to antimicrobial susceptibility testing, conjugation and transformation experiments, and complete genome sequencing.
View Article and Find Full Text PDFDetection of a hydrogen sulfide-negative Typhimurium from cattle feces in a cross-sectional study of cow-calf herds in the Southeastern United States.
Front Vet Sci
July 2025
Center for Food Animal Health, Food Safety and Defense, College of Veterinary Medicine, Department of Pathobiology, Tuskegee University, Tuskegee, AL, United States.
Introduction: Cattle are well-recognized reservoirs of ; however, reports of atypical hydrogen sulfide (HS)-negative strains from bovine sources remain scarce. This cross-sectional study aimed to investigate the antimicrobial resistance profiles and epidemiology of among rural cow-calf herds in Alabama, United States, with a particular focus on isolating emerging HS-negative variants.
Method: Between April and May 2024, a total of 311 fecal samples were collected from cattle across 18 farm operations in six counties.
Unveiling the Genetic Diversity and Antimicrobial Resistance Profiles of Salmonella Population From 2016 to 2020 in Thai Canal Water.
Environ Microbiol Rep
August 2025
Department of Microbiology, Faculty of Public Health, Mahidol University, Bangkok, Thailand.
Salmonella is one of the important pathogens causing acute gastroenteritis, and antimicrobial-resistant Salmonella raises a critical public health concern. Canals in Bangkok, Thailand, play a vital role as sources of agricultural and daily water usage. By employing whole genome sequencing to analyse 351 Salmonella genomes isolated between 2016 and 2020, we expanded the understanding of the characteristics and antimicrobial resistance properties of Salmonella enterica found in Bangkok canals, an underrepresented biome in research.
View Article and Find Full Text PDFAged Turkey manure shapes microbial diversity and antibiotic resistance genes in soil and plants under fertilization.
Sci Rep
July 2025
UNESCO Chair On Ecohydrology and Applied Ecology, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, 90-237, Lodz, Poland.
The growing use of animal manure in agriculture has raised concerns about its impact on soil health and the dissemination of antibiotic resistance genes (ARGs), particularly from less-studied sources like Turkey manure. Despite its widespread use, little is known about how aged Turkey manure affects soil-plant systems. This study investigates its influence on soil physicochemical properties, microbial diversity, and the distribution of ARGs.
View Article and Find Full Text PDFGenomic Insights of Antibiotic-Resistant Isolated from Intensive Pig Farming in South Africa Using 'Farm-to-Fork' Approach.
Antibiotics (Basel)
April 2025
Antimicrobial Research Unit, College of Health Sciences, University of KwaZulu-Natal, Durban 4000, South Africa.
: Intensive pig farming is a critical component of food security and economic activity in South Africa; however, it also presents a risk of amplifying antimicrobial resistance (AMR). This study provides genomic insights into antibiotic-resistant () circulating across the pork production chain, using a 'farm-to-fork' approach. : A total of 417 samples were collected from various points along the production continuum, including the farm (n = 144), transport (n = 60), and abattoir (n = 213).
View Article and Find Full Text PDF