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Diagnosis of infection-causing microorganisms with sensitive, rapid, selective and economical diagnostic tests is critical to start the right treatment. With these tests, the spread of infections can be prevented. In addition to that, the detection of antimicrobial resistance also makes a significant contribution to public health. In recent years, different types of diagnostic tests have been developed as alternatives to traditional diagnostic tests used in clinics. In particular, colorimetric tests, which minimize the need for an instrument, have advantages owing to their cost effectiveness, rapid response and naked-eye detection and practical use. In this review, we especially focused on pH indicators and nanomaterial-based colorimetric tests in detection of infection-causing microorganisms and antimicrobial resistance.
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http://dx.doi.org/10.3390/diagnostics13142427 | DOI Listing |
Mikrochim Acta
September 2025
Hunan Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China.
An Ag-functionalized structural color hydrogel (Ag-SCH) sensor is constructed for colorimetric detection of glutathione (GSH). The hydrogel is prepared by using the coordination of Ag and 1-vinylimidazole (1-VI) as cross-linking network. GSH acts as a competitive ligand to break the coordination between Ag and 1-VI, leading to the expansion and structural color change of the hydrogel.
View Article and Find Full Text PDFMikrochim Acta
September 2025
Department of Surgical Oncology, Shaanxi Provincial People's Hospital, 256 Friendship West Road, Beilin District, Xi'an, 710068, Shaanxi, China.
Mycoplasma pneumonia, a primary aetiological agent of atypical pneumonia, necessitates the implementation of rapid point-of-care diagnostics. Lateral flow immunoassays (LFIAs) hold promise for point-of-care testing (POCT), yet their sensitivity levels are frequently constrained by probe affinity and matrix interference. We introduce an orientational labelling strategy that employs magnetic nanoparticles (MNPs) functionalized with staphylococcal protein A (SPA) to simultaneously enhance antibody orientation and facilitate magnetic enrichment.
View Article and Find Full Text PDFACS Omega
September 2025
Department of Chemistry, Materials and Chemical Engineering "G. Natta", Politecnico di Milano, Via Mancinelli 7, Milan 20131 Italy.
Point-of-Care (PoC) diagnostics are transforming healthcare by enabling rapid and accessible disease detection right at the patient's bedside. This comprehensive review examines recent advances in nucleic acid (NA)-based PoC testing, revealing how these technologies are revolutionizing molecular diagnostics. Here, we critically analyze the three key components of NA-based PoC development: (i) probe design strategies, (ii) immobilization techniques, and (iii) detection methodologies.
View Article and Find Full Text PDFFood Sci Nutr
September 2025
Chemistry Laboratory, Kouba Higher Normal School, Algiers Kouba Algeria.
The objective of this investigation was to assess the biological properties of the leaf's aqueous extract of (PaAE), which is used in conventional medicine for therapeutic purposes of gastric ulcers and abdominal diseases. The content of phenolic and flavonoidic compounds was quantitatively estimated using colorimetric methods. The phenolic component profile was also evaluated using LC-MS/MS.
View Article and Find Full Text PDFAnal Chim Acta
November 2025
College of Chemical Engineering, Xiangtan University, Xiangtan, 411105, China. Electronic address:
Background: Aflatoxin B1 (AFB1) is a highly carcinogenic mycotoxin frequently found in contaminated food products, posing a significant threat to public health and food safety. Therefore, the development of rapid, sensitive, and reliable detection methods for AFB1 is critical for early warning and prevention. However, traditional detection techniques often require expensive equipment, skilled personnel, and complex procedures, limiting their suitability for on-site applications.
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