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Article Abstract

Root-lesion nematodes (genus ) belong to a diverse group of plant-parasitic nematodes (PPN) with a worldwide distribution. Despite being an economically important PPN group of more than 100 species, genome information related to genus is scarcely available. Here, we report the draft genome assembly of generated on the PacBio Sequel IIe System using the ultra-low DNA input HiFi sequencing workflow. The final assembly created using 500 nematodes consisted of 276 decontaminated contigs, with an average contig N50 of 1.72 Mb and an assembled draft genome size of 227.24 Mb consisting of 51,146 predicted protein sequences. The benchmarking universal single-copy ortholog (BUSCO) analysis with 3131 nematode BUSCO groups indicated that 65.4% of the BUSCOs were complete, whereas 24.0%, 41.4%, and 1.8% were single-copy, duplicated, and fragmented, respectively, and 32.8% were missing. The outputs from GenomeScope2 and Smudgeplots converged towards a diploid genome for . The data provided here will facilitate future studies on host plant-nematode interactions and crop protection at the molecular level.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10138993PMC
http://dx.doi.org/10.3390/ijms24087311DOI Listing

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