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Objectives: This study aimed to compare the effectiveness of an experimental root canal irrigant and 17% Ethylene-di-amine tetra acetic acid for removal of the smear layer in the coronal, middle and apical portions of the root canal.
Materials And Methods: Ninety human single rooted maxillary and mandibular teeth were selected for this study. The teeth were randomly divided into two experimental groups and one control group as follows: Group A (Ethanolic extract of Sapindus Mukorossi), Group B (17% EDTA), and Group C (Distilled water). The root canals of all three groups were prepared with stainless steel K-files by means of the standard step-back technique and irrigated with 5.25% sodium hypo chloride. The teeth were decoronated, following the irrigation and divided longitudinally into two-halves and visualized using scanning electron microscope (SEM) for the amount of smear layer present utilizing the three-point score system. The observations were noted both before and after the treatment. Nonparametric tests were applied for the comparison and -value ⩽ 0.05 was considered as statistically significant.
Results: It was evident from that smear layer was completely removed in coronal portion of 27 out of 30 teeth in-group A. For middle and apical areas of group A, 24 and 19 teeth showed complete smear layer removal. In-group B it was found that there were 24, 21, and 3 teeth at coronal, middle and apical, areas respectively where smear layer were completely absent. Intra group comparison showed a significant difference ( = 0.002) in smear layer removal was found for group A at coronal, middle and apical thirds. Similarly, a significant difference ( = 0.001) was also found for group B; however heavy smear layer was found among the three parts of the canal for group C.
Conclusions: Ethanolic extract of Sapindus Mukorossi have higher effectiveness in removing the smear layer from the root canal in comparison to 17% EDTA.
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http://dx.doi.org/10.1177/22808000231166210 | DOI Listing |
J Dent
September 2025
Department of Restorative Dentistry, Faculty of Dentistry, Malaya University, Kuala Lumpur, Malaysia. Electronic address:
Objectives: to evaluate the effect of smear-layer deproteinization using papain gel and SPRG-adhesive on marginal-gap, anti-demineralization of enamel and dentin after chemical pH cycling and assess acid-base resistance zone (ABRZ) characteristics.
Methods: Cylindrical cavities were prepared cervically in thirty-two extracted premolars. Teeth were divided into two pretreatment groups (n=16); deproteinization with papain enzyme gel (Papacarie Due, Brazil) for 60 second, and no-deproteinization.
J Endod
August 2025
Department of Bioscience Research, College of Dentistry, University of Tennessee Health Science Center, Memphis, TN, USA; Department of Endodontics(,) College of Dentistry, The University of Tennessee Health Science Center, Memphis, Tennessee; Department of Physiology, College of Medicine, Universit
Introduction: To investigate the combined use of laser, nanobubble water (NB-HO), and calcium hydroxide (Ca(OH)) for endodontic disinfection, particularly in regenerative endodontic cases where maximum effectiveness is needed.
Methods: Canal of human teeth were prepared, sterilized and inoculated with Enterococcus faecalis (E. faecalis).
BMC Oral Health
August 2025
Department of Periodontology, Faculty of Dentistry, Gazi University, Ankara, Türkiye.
Background: Root surface biomodification (RSB) enhances tissue attachment by removing the smear layer, facilitating collagen fibril formation, and promoting clot formation and stabilization. This study aimed to evaluate the efficacy of injectable platelet-rich fibrin (i-PRF), an autologous blood product, as a potential adjunct to ethylenediaminetetraacetic acid (EDTA) for RSB in gingival fibroblast attachment and proliferation in vitro.
Methods: Dentin discs (4 mm in diameter) underwent root surface debridement to remove damaged cementum.
Shanghai Kou Qiang Yi Xue
June 2025
Department of Stomatology, Chifeng University Affiliated Hospital. Chifeng 024000, China. E-mail:
Purpose: To evaluate the effects of Er:YAG laser combined with NaClO on the removal of root canal taint layer, calcium hydroxide clearance and dentin microhardness.
Methods: A total of 318 single-rooted extracted teeth collected from March 2021 to January 2024 were selected, the 13 mm root was taken and mechanically prepared and randomly divided into three groups, with 106 teeth in each group. The control group was rinsed with NaClO, the experimental group 1 was rinsed with NaClO combined with ultrasonic washing, and the experimental group 2 was treated with Er:YAG laser on the basis of NaClO.
J Conserv Dent Endod
August 2025
Department of Orthodontics and Dentofacial Orthopedics, Bharati Vidyapeeth (Deemed to be University) Dental College and Hospital, Pune, India.
Aim: The aim of the study was to evaluate the antimicrobial properties of calcium hydroxide (CH)-loaded polylactic-co-glycolic acid (PLGA) nanoparticles (NPs) and CH when used as an intracanal medicament (ICM).
Methods: Adhering guidelines of the Preferred Reporting Items for Systematic Reviews and Meta-analysis, the review was registered in the Prospective Registration of Systematic Review-CRD42023454265. Databases from January 2000 to December 2023 were examined for studies evaluating the various properties of CH-loaded PLGA NPs compared to conventional ICM-CH.