Ellagitannins from Mill. Leaf Extracts Impair Viability and Infection-Induced Inflammation in Human Gastric Epithelial Cells.

() is an etiologic factor of peptic ulcer disease and gastric cancer. Virulent strains of are correlated with the severity of gastritis, due to NF-κB activation and IL-8 expression at the epithelial level. Ellagitannins have been documented for antibacterial and anti-inflammatory activities, thus suggesting their potential use in gastritis. Recently, several authors, including our group, demonstrated that tannin-rich extracts from chestnut byproducts, at present considered agricultural waste, display promising biological activities. In this work, we detected high levels of polyphenols in hydroalcoholic extracts from chestnut leaves ( L.). Among polyphenols, the ellagitannin isomers castalagin and vescalagin (about 1% of dry extract) were identified as potential bioactive compounds. In GES-1 cells infected by , leaf extract and pure ellagitannins inhibited IL-8 release (IC ≈ 28 µg/mL and 11 µM, respectively). Mechanistically, the anti-inflammatory activity was partly due to attenuation of NF-κB signaling. Moreover, the extract and pure ellagitannins reduced bacterial growth and cell adhesion. A simulation of the gastric digestion suggested that the bioactivity might be maintained after oral administration. At the transcriptional level, castalagin downregulated genes involved in inflammatory pathways (NF-κB and AP-1) and cell migration (Rho GTPase). To the best of our knowledge, this is the first investigation in which ellagitannins from plant extracts have demonstrated a potential role in the interaction among and human gastric epithelium.