Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Necrotizing enterocolitis (NEC) is a deadly gastrointestinal disease of premature infants that is associated with an exaggerated inflammatory response, dysbiosis of the gut microbiome, decreased epithelial cell proliferation, and gut barrier disruption. We describe an in vitro model of the human neonatal small intestinal epithelium (Neonatal-Intestine-on-a-Chip) that mimics key features of intestinal physiology. This model utilizes intestinal enteroids grown from surgically harvested intestinal tissue from premature infants and cocultured with human intestinal microvascular endothelial cells within a microfluidic device. We used our Neonatal-Intestine-on-a-Chip to recapitulate NEC pathophysiology by adding infant-derived microbiota. This model, named NEC-on-a-Chip, simulates the predominant features of NEC, including significant upregulation of proinflammatory cytokines, decreased intestinal epithelial cell markers, reduced epithelial proliferation, and disrupted epithelial barrier integrity. NEC-on-a-Chip provides an improved preclinical model of NEC that facilitates comprehensive analysis of the pathophysiology of NEC using precious clinical samples. This model is an advance toward a personalized medicine approach to test new therapeutics for this devastating disease.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10243823 | PMC |
| http://dx.doi.org/10.1172/jci.insight.146496 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Modeling human retinal ganglion cell axonal outgrowth, development, and pathology using pluripotent stem cell-based microfluidic platforms.
Proc Natl Acad Sci U S A
September 2025
Department of Medical and Molecular Genetics, Indiana University School of Medicine, Indianapolis, IN 46202.
Retinal ganglion cells (RGCs) are highly compartmentalized neurons whose long axons serve as the sole connection between the eye and the brain. In both injury and disease, RGC degeneration occurs in a similarly compartmentalized manner, with distinct molecular and cellular responses in the axonal and somatodendritic regions. The goal of this study was to establish a microfluidic-based platform to investigate RGC compartmentalization in both health and disease states.
View Article and Find Full Text PDFTunable cell separation using a thermo-responsive deterministic lateral displacement device.
Lab Chip
September 2025
Institute of Integrated Research, Institute of Science Tokyo, R2-9, 4259 Nagatsuta-cho, Midoriku, Yokohama, Kanagawa 226-8501, Japan.
Tunability in isolating target cells of varying sizes from complex heterogeneous samples is essential for biomedical research and diagnostics. However, conventional deterministic lateral displacement (DLD) systems lack flexibility due to their fixed critical diameters (). Here, we present a thermo-responsive DLD micropillar array that enables tunable cell separation by dynamically modulating through temperature control.
View Article and Find Full Text PDFAdvanced strategies in organoid/organ-on-a-chip for food safety and nutrition.
Food Res Int
November 2025
Medical School of Nantong University, Nantong 226001, China. Electronic address:
Food nutrition and safety are fundamental to the food industry, and the development of appropriate research models is crucial. Unlike traditional animal models, the innovative organoid/organ-on-a-chip model possess distinct human-like characteristics and genomic stability, which have garnered significant attention in food research. In this review, we conduct a comparative analysis between organoids and traditional animal and 2D cell models.
View Article and Find Full Text PDFProtective effect of osmanthus water extract on liver dysfunction caused by DBP based on organoids and organ chips technologies.
Food Res Int
November 2025
Key Laboratory of Environmental Related Diseases and One Health, Xianning Medical College, Hubei University of Science and Technology, Xianning 437100, China. Electronic address:
This study aimed to investigate the protective mechanism of Osmanthus fragrans water extract (OSF) against liver injury induced by dibutyl phthalate (DBP). We utilized liver organoids and liver organ chip technology to replicate the liver microenvironment in vivo. Metabolomic analysis revealed that DBP induced oxidative stress and lipid metabolism disorders; however, following intervention with OSF, the associated abnormal metabolites were significantly reduced.
View Article and Find Full Text PDFAn electrochemiluminescence device powered by streaming potential for the detection of amines in flowing solution.
Nat Commun
September 2025
Department of Chemical Science and Engineering, School of Materials and Chemical Technology, Institute of Science Tokyo, Nagatsuta-cho, Midori-ku, Yokohama, Japan.
The research and implementation of portable and low-cost analytical devices that possess high reproducibility and ease of operation is still a challenging task, and a growing field of importance, within the analytical research. Herein, we report the concept, design and optimization of a microfluidic device based on electrochemiluminescence (ECL) detection that can be potentially operated without electricity for analytical purposes. The device functions exploiting the concept of streaming potential-driven bipolar electrochemistry, where a potential difference, generated from the flow of an electrolyte through a microchannel under the influence of a pressure gradient, is the driving force for redox reactions.
View Article and Find Full Text PDF